摘要
目的分析结直肠癌组织中周期素依赖性蛋白激酶抑制物1A相互作用锌指蛋白1(Ciz1)的表达情况并探讨其在临床诊断和治疗中的潜在意义。方法采用免疫组化技术和Western blot法,对结直肠癌组织、正常癌旁结直肠组织中Ciz1表达水平进行检测、对比。结果结直肠癌组织原发灶表中,Ciz1明显增加,阳性率与正常组织比较,差异有统计学意义(P<0.05)。采用Western blot法定量分析,结果显示结直肠癌组织Ciz1的表达量为0.32±0.03,正常大肠黏膜组织中的表达量为0.11±0.01,二者间差异有统计学意义(P<0.05)。Ciz1在结直肠癌组织中过度表达与多个临床病理特征密切相关。患者的年龄、性别、肿瘤部位、分化程度,以及是否存在远处转移与此并无关联(均P>0.05)。结直肠癌组织中Ciz1的表达水平升高,与结直肠癌的T分期(P=0.018)、淋巴结转移(P=0.022)以及美国癌症联合委员会(American Joint Committee on Cancer,AJCC)分期(P=0.017)密切相关。结论Ciz1在肿瘤分期过程中参与了肿瘤的发展和扩散。可以预见Ciz1有望成为一项新的标志物,用于评估结直肠癌患者的预后情况。
Objective To observe the expression of ciz1 in human colorectal cancer tissues and analyse the relationship between their expression and clinicopathologic features.Methods We detected the expression of Ciz1 and Ciz1 mRNA by immunohistochemistry and Western blotting.The relationship between the expression of ciz1 and clinicopathologic features was analied.Results According to immunohistochemical results,Ciz1 showed significant high expression in the primary lesion of colorectal cancer tissue,compared to normal adjacent tissues(66.7%vs.35.0%,P=0.001).Through Western blot analysis,it was found that the relative expression level in colorectal cancer tissue was 0.32±0.03,while in normal colorectal mucosal tissue it was 0.11±0.01.In addition,the relative expression level of Ciz1 in colorectal cancer tissue was significantly higher than that in normal intestinal mucosal tissue(P<0.05).The study found that the overexpression of Ciz1 in colorectal cancer tissue is significantly correlated with the T stage(P=0.018),lymph node metastasis(P=0.022),and AJCC stage(P=0.017)of the cancer.The age,gender,tumor location,degree of differentiation,and the presence of distant metastasis of patients were not correlated with this(P>0.05).The expression level of Ciz1 in colorectal cancer tissue is significantly increased,which is closely related to the T stage(P=0.018),lymph node metastasis(P=0.022),and American Joint Committee on Cancer stage(P=0.017)of colorectal cancer.Conclusion This association suggests that Ciz1 may play an important role in tumor staging,participating in the development and spread of tumors.Therefore,it can be foreseen that Ciz1 is expected to become a new biomarker for evaluating the prognosis of colorectal cancer patients.
作者
曹金
高芳园
孙胜波
张焕虎
韩传吉
宫庆
李光兴
CAO Jin;GAO Fangyuan;SUN Shengbo;ZHANG Huanhu;HAN Chuanji;GONG Qing;LI Gangxing(Department of Gastrointestinal Surgery,Weihai Municipal Hospital Affiliated to Shandong University,Weihai 264202,Shandong,China)
出处
《中国现代医生》
2024年第23期78-80,86,共4页
China Modern Doctor
关键词
Ciz1
结直肠癌
免疫组化
Ciz1
Colorectal cancer
Immunohistochemical
