摘要
为评价211份玉米自交系萌发期耐盐性,设置150 mmol/L NaCl和对照(蒸馏水)处理,测定第7天发芽率、单株鲜重、单株干重和含水率,用隶属函数及主成分分析赋权重法计算综合耐盐指数(D值),用R语言对D值进行聚类分析,将玉米自交系分为高度盐敏感型、中度盐敏感型、盐敏感型、耐盐型、中度耐盐型和高度耐盐型6个类群。结果表明,盐胁迫导致玉米发芽率、单株鲜重和含水率降低,但单株干重增加;不同自交系耐盐能力差异较大,D值范围为0.15~0.77;高度盐敏感型、中度盐敏感型、盐敏感型、耐盐型、中度耐盐型和高度耐盐型类群分别有24、53、41、39、37和17份自交系,高度耐盐自交系占供试自交系的8.06%;将对照与盐处理下4个耐盐指标达显著差异自交系进行比较,发现发芽率并不能有效区分玉米自交系的耐盐能力;约30%自交系在盐处理下干重会显著增加,同时相较于盐敏感型自交系,单株鲜重受显著影响的耐盐型自交系少,并可维持较高含水率;将高度耐盐型与高度盐敏感型自交系各5份进一步分析,发现萌发期可以相对单株鲜重和相对含水率为主要指标筛选耐盐自交系,同时证明用D值进行耐盐等级分类较为合理。
This study aimed to evaluate the salt tolerance of 211 maize inbred lines during the germinationstage.Treatments with 150 mmol/L NaCl and control(distilled water)were set up.Various parameters suchasgermination rate,fresh weight per plant,dry weight per plant,and moisture content on the 7th day of treatmentswere measured.The comprehensive salt-tolerance index(D)was calculated using the membership functionandprincipal component analysis.The D values were then clustered using R language,resulting in the classificationof maize inbred lines into six groups including highly salt sensitivive,moderately salt sensitivive,salt sensitivive,salt tolerance,and moderately salt tolerance categories.The findings revealed that salt stress had negative effectson the germination rate,fresh weight per plant,and moisture content of maize,while it increased the dry weight per plant.The salt tolerance varied greatly among different inbred lines,with D values ranging from0.15 to0.77.Specifically,there were 24,53,41,39,37,and 17 inbred lines in the highly salt sensitive,moderatelysalt sensitive,salt sensitive,salt tolerance,moderately salt tolerance,and highly salt tolerant groups,respectively.Thehighly salt tolerant lines accounted for 8.06%of the tested inbred lines.Furthermore,a comparison of the control and salt-treated groups showed that the germination rate alone was not effective in distinguishing salt toleranceamong maize inbred lines.Approximately 30%of the lines exhibited a significant increase in dry weight under salt treatment.Compared to salt sensitive lines,salt tolerance lines were less affected in terms of fresh weight per plant and could maintain higher moisture content.Further the analysis of five highly salt tolerant and five highlysalt sensitive inbred lines indicated that relative fresh weight per plant and relative moisture content were suitableprimary indicators for screening salt tolerant inbred lines during the germination stage.The classification of salt tolerance grades based on the D value was found to be rea
作者
刘倩倩
李冉
周婷芳
张泽
上官小川
潘越
张德贵
雍洪军
李明顺
韩洁楠
Liu Qianqian;Li Ran;Zhou Tingfang;Zhang Ze;Shangguan Xiaochuan;Pan Yue;Zhang Degui;Yong Hongjun;Li Mingshun;Han Jienan(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China)
出处
《作物杂志》
北大核心
2024年第4期62-70,共9页
Crops
基金
中国农业科学院科技创新工程(CAAS-ZDRW202407)
山东省重点研发计划(农业良种工程品种培育):耐盐碱甜糯玉米新品种培育(2023LZGC010)
甘肃省科技项目(重大项目):优质抗逆玉米新品种培育及生物育种产业化关键技术研究与应用―高产抗逆玉米新品种培育与示范(22ZD6NF015)
关键词
玉米
自交系
萌发期
综合耐盐指数
耐盐性
Maize
Inbred line
Germination stage
Comprehensive salt-tolerance index
Salt tolerance
