摘要
目的:观察蒙药香青兰总黄酮(total flavones of dracocephalum moldavica,TFDM)对大鼠脑缺血再灌注损伤后缺血半暗带区血管内皮生长因子2(vascular endothelial growth factor 2,VEGFR2)表达的影响,探讨其对血管生成的作用。方法:SD大鼠线栓法制备大脑中动脉闭塞再灌注模型,TFDM 50 mg/kg灌胃给药,在手术后第1、3、7、14天4个时相进行取材,免疫组织化学染色观测VEGFR2阳性标记的细胞及新生血管在缺血半暗带区的分布情况,Western blot检测缺血半暗带区VEGFR2的表达情况。结果:免疫组织化学染色结果显示,再灌注术后第3~14天时,TFDM给药组阳性细胞表达数量显著高于模型组(P<0.05),且在微血管、微动脉和微静脉附近表达增多;Western blot检测结果也显示,TFDM给药组VEGFR2表达上调。结论:TFDM可能通过上调VEGFR2蛋白的表达,促进脑缺血半暗带区微血管的生成,改善微循环功能。
Objective:To observe the effect of Mongolian medicine total flavones of Dracocephalum moldavica L.(TFDM)on the expression of vascular endothelial growth factor 2(VEGFR2)in ischemic penumbra of rats after cerebral ischemia-reperfusion,and to explore its effect on angiogenesis.Methods:The middle cerebral artery occlusion(MCAO)reperfusion model of SD rats was prepared by suture method.TFDM 50 mg/kg was administered by intragastric administration.The samples were taken at 1,3,7 and 14 days after operation.Immunohistochemical staining was used to observe the distribution of VEGFR2 positive labeled cells and neovascularization in the ischemic penumbra.Western blot was used to detect the expression of VEGFR2 in the ischemic penumbra.Results:Immunohistochemical staining showed that the number of positive cells in the TFDM administration group on the 3rd to 14th day after MCAO reperfusion was significantly higher than that in the model group(P<0.05),and the expression increased near the microvessels,arterioles and venules.Western blot also showed that the expression of VEGFR2 was up-regulated in TFDM group.Conclusion:TFDM may upregulate the expression of VEGFR2 protein,promote the generation of microvessels in the ischemic penumbra,and improve microcirculation function.
作者
高天一
翟维凯
蔡志平
崔成立
李梁
GAO Tianyi;ZHAI Weikai;CAI Zhiping;CUI Chengli;LI Liang(School of Basic Medicine and Forensic Medicine,Baotou Medical College,Baotou 014040,China;Department of Human Anatomy,School of Basic Medicine and Forensic Medicine of Baotou Medical College)
出处
《包头医学院学报》
CAS
2024年第8期86-90,共5页
Journal of Baotou Medical College
基金
内蒙古自治区高等学校科学技术研究项目(NJZY19198)。
关键词
香青兰总黄酮
脑缺血再灌注
血管内皮生长因子受体2
血管生成
缺血半暗带区
Total flavones of Dracocephalum moldavica L.
Cerebral ischemia-reperfusion
Vascular endothelial growth factor receptor 2
Angiogenesis
Ischemic penumbra area
