摘要
本研究旨在探究HEV感染状态下兔粪便菌群的变化差异。本研究收集粪便HEV RNA阳性与阴性兔粪便各4份,分为P组(n=4,P1~P4)和N组(n=4,N1~N4),对粪便16S rDNA基因序列中的V3V4区域进行高通量测序,并对所得数据进行了生物信息学分析。α多样性及PCoA分析结果显示2组间的粪便菌群组成存在明显差异;与阴性对照组相比,HEV感染组的拟杆菌门、酸杆菌门、绿弯菌门等显著增多(P<0.05),瘤胃球菌属、拟杆菌属等丰度显著减少(P<0.05)。HEV感染后兔粪便菌群的构成、丰度等均存在一定差异,粪便菌群有可能成为HEV感染的潜在生物标志物。
This study was designed to investigate the changes in fecal microbiota in rabbits with Hepatitis E virus(HEV)infection.A total of 4 fecal samples of HEV RNA positive rabbits and 4 fecal samples of HEV RNA negative rabbits were collected and divided into P and N groups.The next-generation 16S rDNA sequencing was performed and the data was analyzed for these changes.The results of alpha diversity and PCoA analysis showed a significant difference in the compositions of fecal microbiota between the two groups.Compared with the negative control group,Bacteroidota,Acidobacteriota,Chloroflexi and Muribaculaceae in the HEV infection group significantly increased(P<0.05)while Ruminococcus and Bacteroides significantly decreased(P<0.05).These changes in the composition and abundance of fecal microbiota in rabbits with HEV infection might have the potential significance to become a biomarker of HEV infection.
作者
李曼郁
蓝海云
李克坚
周诚
LI Manyu;LAN Haiyun;LI Kejian;ZHOU Cheng(Division I of In Vitro Diagnostics for Infectious Diseases,Institute for In Vitro Diagnostics Control,National Institutes for Food and Drug Control,Beijing 100050,China)
出处
《中国动物传染病学报》
CAS
北大核心
2024年第4期125-131,共7页
Chinese Journal of Animal Infectious Diseases
关键词
戊型肝炎病毒
粪便菌群
高通量测序
Hepatitis E virus
fecal microbiota
high-throughput sequencing
