摘要
目的探讨沉默信息调节因子6(Sirtuin 6,SIRT6)在胃癌组织中的表达及临床意义。方法生物信息学分析SIRT6 mRNA在胃癌中的表达及其与生存、肿瘤突变负荷(TMB)及TIDE评分的关系;收集95例胃癌及癌旁组织标本,免疫组织化学方法检测SIRT6的表达水平,分析其与临床病理特征的关系;Western blot法检测10例新鲜胃癌及癌旁组织中SIRT6的表达情况。结果生物信息学分析显示,SIRT6 mRNA在胃癌中表达高于正常胃组织(P<0.05),Kaplan-Meier生存曲线显示SIRT6高表达组生存时间较短,突变相关分析及TIDE评分显示SIRT6高表达组具有更高的TMB及TIDE评分(P<0.05),且SIRT6 mRNA表达水平与TMB及TIDE评分呈显著正相关(P<0.05)。免疫组织化学结果显示SIRT6在胃癌组织中表达率为85.2%(81/95);SIRT6在胃癌中高表达与高Ki-67增殖指数相关,差异具有统计学意义(P<0.05),SIRT6表达在不同年龄、性别、肿瘤直径及P53等组间差异均无统计学意义(P>0.05);Western blot显示胃癌组织中SIRT6的表达水平高于癌旁组织(9/10),差异具有统计学意义(P<0.05)。结论SIRT6在胃癌组织中高表达,可能通过影响基因组稳定性及胃癌细胞增殖能力参与胃癌的发生发展。
Objective To investigate the expression and clinical significance of Sirtuin 6(SIRT6)in gastric cancer tissues.Methods Bioinformatics was used to analyze SIRT6 mRNA expression in gastric cancer and its relationship with survival,tumor mutational burden(TMB)and TIDE score.Ninety-five cases of gastric cancer and adjacent tissue samples were collected,the expression level of SIRT6 was detected by immunohistochemistry,and the relationship between SIRT6 and clinicopathologic features was analyzed.SIRT6 expression in 10 cases of fresh gastric cancer and adjacent tissues was detected by Western blotting.Results Bioinformatics analysis showed that SIRT6 mRNA expression in gastric cancer was higher than that in normal gastric tissues(P<0.05).Kaplan-Meier survival curve showed that the survival time of the group with high SIRT6 expression was shorter.Mutation correlation analysis and TIDE score showed that SIRT6 high expression group had higher TMB and TIDE score(P<0.05),and SIRT6 mRNA expression level was significantly positively correlated with TMB and TIDE score(P<0.05).Immunohistochemical results showed that the expression rate of SIRT6 in gastric cancer tissues was 85.2%(81/95).The high expression of SIRT6 in gastric cancer was correlated with high Ki-67 proliferation index,and the difference was statistically significant(P<0.05),while there were no significant differences in SIRT6 expression among different age,gender,tumor diameter and P53 groups(P>0.05).Western blotting showed that the expression level of SIRT6 in gastric cancer tissues was higher than that in adjacent tissues(9/10),and the difference was statistically significant(P<0.05).Conclusion SIRT6 is highly expressed in gastric cancer tissues,which may be involved in the occurrence and development of gastric cancer by affecting the genomic stability and the proliferation ability of gastric cancer cells.
作者
佟美辰
周而乐
王思佳
曹璋
TONG Meichen;ZHOU Erle;WANG Sijia;CAO Zhang(Department of Pathology,Binzhou Medical University Hospital,Binzhou 256603,Shandong,P.R.China;School of Basic Medicine,Binzhou Medical University,Yantai 264003,Shandong,P.R.China)
出处
《滨州医学院学报》
2024年第4期268-273,共6页
Journal of Binzhou Medical University
基金
山东省自然科学基金(ZR2020MH243)。
