摘要
为揭示盐胁迫下不同核桃基因型的适应机制及耐盐性差异,筛选耐盐性较强的种资资源,研究了盐胁迫下3种不同基因型核桃幼苗叶、根解剖结构和营养元素的变化。以北加州黑核桃(J1)、杂种核桃‘中宁异’(J2)、‘新新2号’核桃(J3)一年生实生幼苗为材料,在盆栽条件下进行NaCl胁迫(0、50、100、200 mmol/L),研究幼苗叶、根解剖结构和Na、K、Ca、Mg、Fe、Cu、Zn含量的变化及其吸收、运输和分配特征。结果表明:盐胁迫下,J1的叶片、上表皮、下表皮、栅栏组织和海绵组织的厚度、根维管束直径下降;J2的叶片、上表皮、下表皮、栅栏组织、海绵组织和皮层的厚度、栅海比、组织紧密度、维管束和导管的直径、皮层厚度占径比随盐浓度的增加呈先增加后降低趋势;盐处理下,J3的叶片、下表皮、栅栏组织的厚度、栅海比、皮层厚度占径比下降。随盐浓度的增加,Na含量呈上升趋势,在200 mmol/L下大幅度增加,J1、J2、J3叶中Na增幅为412.00%、130.05%、577.08%;K、Ca、Mg、Fe、Cu、Zn的吸收受到盐胁迫影响,K/Na、Ca/Na、Mg/Na降低,其中J2叶的K/Na、Mg/Na降幅最小,营养元素转运能力高于J1和J3。核桃幼苗通过加强根输导组织对K、Ca、Mg的选择性吸收和运输能力,维持叶片的基本结构与功能,从而抵御盐胁迫。主成分分析与隶属函数相结合得出耐盐强弱依次为杂种核桃‘中宁异’>‘新新2号’核桃>北加州黑核桃。
The anatomical structure and nutrient content of three genotypes of walnut seedlings under salt stress were studied to reveal the response and adaptation mechanisms of salt stress and the differences in salt tolerance of different varieties.The annual seedlings of Northern California black walnut(Juglans hindsii)(J1),‘Zhongningyi’(Juglans major×Juglans regia)(J2),‘Xinxin No.2’(Juglans regia)(J3)were used as materials.The annual seedlings were treated with salt stress(0,50,100,200 mmol/L NaCl)under pot conditions to study the microanatomical parameters of leaves and roots,and the changes of Na,K,Ca,Mg,Fe,Cu,Zn contents and their absorption,transportation and distribution characteristics in roots and leaves.The results show that:under salt stress,the thickness of leaves,upper epidermis,lower epidermis,palisade tissue,sponge tissue and root cortex,as well as the diameter of the root vascular bundle decreased in J1.The thickness of leaves,upper epidermis,lower epidermis,palisade tissue,sponge tissue and root cortex,the ratio of palisade tissue thickness to spongy tissue thickness,tissue tightness,diameter of root vascular bundle and root vessel,and the ratio of root cortex thickness to root diameter of J2 increased first and then decreased with the increase of salt concentration.Under salt stress,the thickness of leaves,lower epidermis,palisade tissue,the ratio of palisade tissue thickness to spongy tissue thickness,and the ratio of root cortex thickness to root diameter of J3 decreased.With the increase of salt concentration,the Na content showed an upward trend,which increased significantly at 200 mmol/L,and the Na content in J1,J2 and J3 leaves increased by 412.00%,130.05%and 577.08% compared with the control.The absorption,transportation and distribution characteristics in roots and leaves of K,Ca,Mg,Fe,Cu and Zn were affected by salt stress,and K/Na,Ca/Na and Mg/Na decreased under salt stress,among which the decrease of Na/K and Mg/Na in J2 leaves was the smallest.The nutrient transport capacity of J1 w
作者
唐佳莉
姬新颖
李敖
郑旭
张俊佩
TANG Jiali;JI Xinying;LI Ao;ZHENG Xu;ZHANG Junpei(State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland Administration,Chinese Academy of Forestry,Beijing 100091,China)
出处
《生态学报》
CAS
CSCD
北大核心
2024年第15期6795-6810,共16页
Acta Ecologica Sinica
基金
国家重点研发计划(2022YFD2200402)。
关键词
盐胁迫
核桃
解剖结构
营养元素
K/Na
salt stress
walnut
anatomical structure
nutrient elements
K/Na
