摘要
针对目前初始荧光检测技术集中在特征吸收波长领域,非特征吸收波长检测灵敏度低,难以准确反映藻类捕光色素能量吸收效率的问题,提出了荧光动力学法浮游植物初级生产力测量中的初始荧光准确获取技术。利用OJIP荧光动力学技术测量原理调控电子受体氧化还原状态,获得最佳激发条件,利用积分放大技术对多波段初始荧光进行高灵敏检测。测量结果表明:初始荧光能够准确反映藻类色素的实际吸收情况,铜绿微囊藻在569 nm出现藻红蛋白(PE)吸收峰,在620 nm出现藻蓝蛋白(PC)主要吸收峰,4种淡水绿藻均在439 nm出现Chl a吸收峰,在474 nm出现Car吸收峰;5种受试藻种的荧光产率F_(PSII)与初始荧光相似度均在0.996以上,表明初始荧光能够用于反映光系统Ⅱ(PSII)吸收,且测量准确性较高。
Objective After illumination,the electron transport chain of dark-adapted phytoplankton is inhibited to bring about the gradual closure of the reaction center.Light energy absorbed by the light-harvesting pigment is released solely by fluorescence or thermal dissipation to elevate fluorescence yield.This increase initiates the chlorophyll fluorescence induction process.In the early stage of chlorophyll fluorescence induction,the photochemical reaction has not yet commenced,and the photosynthetic reaction center remains fully accessible.This stage is termed the initial fluorescence phase,and it is measured by light sources with varying wavelengths within the visible light spectrum.These measurements provide vital photosynthetic insights,including pigment content,reaction center concentration,energy absorption,and excitation energy transfer.They precisely depict the structure and composition of light-harvesting pigments in phytoplankton,along with energy absorption efficiency.Consequently,this technique critically contributes to analyzing the photosynthetic status and primary productivity of live phytoplankton.Following the revelation of chlorophyll fluorescence induction,multiple techniques for measuring initial fluorescence have emerged.For example,Schreiber et al.introduced the technique of pulse amplitude modulation(PAM)for measuring photoinduced fluorescence kinetics,Kolber et al.suggested the fast repetition rate fluorescence(FRRF)measurement technique,and Strasser et al.developed the OJIP technique for rapidly measuring chlorophyll fluorescence-induced kinetic curves by continuous excitation luminescence.Currently,research on the technical approaches predominantly centers on characteristic absorption bands.Nevertheless,the sensitivity of non-characteristic absorption bands remains limited,hindering the accurate portrayal of the structural composition of light-harvesting pigments and energy absorption efficiency.Therefore,the development of a profoundly sensitive method for initial fluorescence measurement is
作者
董鸣
殷高方
赵南京
王翔
张小玲
马明俊
梁天泓
贾仁庆
徐敏
胡翔
黄朋
Dong Ming;Yin Gaofang;Zhao Nanjing;Wang Xiang;Zhang Xiaoling;Ma Mingjun;Liang Tianhong;Jia Renqing;Xu Min;Hu Xiang;Huang Peng(School of Environment Science and Optoelectronic Technology,University of Science and Technology of China,Hefei 230026,Anhui,China;Key Laboratory of Environmental Optics and Technology,Anhui Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Hefei 230031,Anhui,China;School of Electrical Engineering,Anhui Polytechnic University,Wuhu 241200,Anhui,China;Institutes of Physical Science and Information Technology,Information Materials and Intelligent Sensing Laboratory of Anhui Province,Anhui University,Hefei 230601,Anhui,China)
出处
《光学学报》
EI
CAS
CSCD
北大核心
2024年第12期11-17,共7页
Acta Optica Sinica
基金
国家重点研发计划(2021YFC3200100,2022YFC3103900,2016YFC1400600)
国家自然科学基金(62005001,42206198)
安徽省科技重大专项(202003a07020007,202203a07020002)。
关键词
海洋光学
初始荧光
荧光动力学
OJIP
高灵敏
ocean optics
initial fluorescence
fluorescence dynamics
OJIP
high sensitivity
