摘要
OJIP荧光动力学被广泛应用于藻类光合作用研究,而在常用的OJIP曲线分析中,常将J、I点的特征时间固定,忽略了藻类种类对J、I点特征时间的影响,这会造成J、I点荧光强度计算结果的偏差,直接影响测量结果的准确性。鉴于此,提出使用三级指数函数逼近OJIP曲线的方法动态获取J、I点的特征时间。不同藻种的J、I点的特征时间和二氯苯基二甲脲(DCMU)胁迫下J点特征时间实验的测试结果表明:所提方法能够有效获得不同藻类J、I点的特征时间,二形栅藻、蛋白核小球藻、普通小球藻和新月筒柱藻J点的特征时间分别为2.22,1.52,1.33,1.01 ms,I点的特征时间分别为28.80,27.15,29.90,15.28 ms,多次计算结果的相对标准偏差均小于10%,具有较好的一致性;在DCMU浓度(质量浓度)分别为10,20,40μg/L的毒性胁迫实验条件下,所提方法计算的普通小球藻的J点特征时间分别为1.25,1.18,1.10 ms,相对标准偏差为12.03%。此外,利用所提方法计算得到的光合活性参数V_(J)与DCMU浓度之间具有良好的毒性剂量-效应关系,相关性系数R^(2)为0.991。
OJIP fluorescence kinetics is widely used in the study of algae photosynthesis,but in common OJIP curve analysis,the characteristic time of J and I points is often fixed.Therefore,the influence of algae species on the characteristic time of J and I points is ignored,which results in the deviation of the fluorescence intensity calculation results of J and I points and directly affects the accuracy of the measurement results.In view of this,a method of using three-level exponential function to approximate OJIP curve is proposed to dynamically obtain the characteristic time of J and I points.The test results of experiments of characteristic time of J and I points of different algae species,and the characteristic time of J point under dichlorophenyl dimethylurea(DCMU)stress show that the proposed method can effectively obtain the characteristic time of J and I points of different algae.The characteristic time of J point of Scenedesmus dimorpha,Chlorella pyrenoidosa,Chlorella vulgaris and Cylindrotheca closterium is 2.22,1.52,1.33,1.01 ms,respectively.The corresponding characteristic time of I point is 28.80,27.15,29.90,15.28 ms,respectively.The relative standard deviation of multiple calculation results is less than 10%,which has a great consistency.Under the toxic stress test conditions with DCMU concentrations(mass concentrations)of 10,20,40μg/L,the characteristic time of J point of Chlorella vulgaris calculated by the proposed method is 1.25,1.18,and 1.10 ms,respectively,and the relative standard deviation is 12.03%.In addition,the photosynthetic activity parameter V_(J) calculated by the proposed method has a good toxic dose-effect relationship with the DCMU concentration,and the correlation coefficient R^(2) is 0.991.
作者
丁志超
殷高方
赵南京
甘婷婷
亓培龙
谷梦园
陈敏
王璐
董鸣
黄朋
马明俊
方丽
杨瑞芳
Ding Zhichao;Yin Gaofang;Zhao Nanjing;Gan Tingting;Qi Peilong;Gu Mengyuan;Chen Min;Wang Lu;Dong Ming;Huang Peng;Ma Mingjun;Fang Li;Yang Ruifang(Key Laboratory of Environmental Optics and Technology,Anhui Institute of Optics and Fine Mechanics,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei,Anhui 230031,China;University of Science and Technology of China,Hefei,Anhui 230026,China;Key Laboratory of Optical Monitoring Technology for Environment,Anhui Province,Hefei,Anhui 230031,China;School of Biological Food and Environment,Hefei University,Hefei,Anhui 230601,China)
出处
《光学学报》
EI
CAS
CSCD
北大核心
2022年第8期241-246,共6页
Acta Optica Sinica
基金
国家重点研发计划项目(2021YFC3200100)
国家自然科学基金项目(61875207)
安徽省科技重大专项(202003A07020007)
安徽省杰出青年科学基金项目(1908085J23)
中科院仪器设备功能开发技术创新项目(后资助)(Y93H3G1251)
中国科学院合肥物质科学研究院院长基金(YZJJZX202013)。
关键词
物理光学
荧光动力学
特征时间确定
指数分析
藻类
physical optics
fluorescence kinetics
characteristic time determination
exponential analysis
algae
