摘要
目的:本研究旨在应用自制给药套管植入大鼠小脑延髓池(CMC),以实现给清醒大鼠多次给药。方法:自制给药套管,从大鼠颅骨枕外嵴中点开孔,沿枕骨内壁下行直至套管尖端达CMC,然后颅钉和自凝树脂固定。应用脂多糖(LPS)作为局部炎性刺激物注射到CMC诱发神经炎症,并使用X线、行为学检测及免疫荧光染色评估该方法的可行性。结果:X线及滂胺天蓝染料确认脑内置管埋置位置合适。经给药管注射LPS组大鼠较Control组大鼠面部机械痛阈值降低(P<0.001);LPS组大鼠在旷场中央停留时间较Control组大鼠显著下降(P<0.01)。免疫荧光染色结果表明LPS激活三叉神经脊束核尾侧亚核(SpVc)小胶质细胞。结论:该方法可有效作用于大鼠SpVc,适用于清醒大鼠小脑延髓池内的重复多次给药。
Objective:This study introduces a novel approach utilizing a self-made drug delivery cannula implanted into the cerebellomedullary cistern(CMC)of rats to allow repeated administrations in conscious subjects.Methods:A self-made medication cannula is inserted through a drilled hole at the midpoint of the occipital crest of the rat’s skull,descending along the inner wall of the occipital bone until reaching the CMC,and securing it in place with skull screws and self-curing resin.Lipopolysaccharide(LPS)is injected into the CMC to induce neuroinflammation,and the feasibility of this method is assessed using X-ray imaging,behavioral testing,and immunofluorescence staining.Results:The placement of the brain cannula was confirmed using X-ray film and pontamine sky blue staining.Rats in the LPS group exhibited a lower facial mechanical pain threshold compared to the Control group(P<0.001),along with reduced residence time in the open field center(P<0.01).Immunofluorescence staining revealed LPS-induced activation of caudal spinal trigeminal nucleus(SpVc)microglia.Conclusion:This method proves to be suitable for multiple administrations to the cerebellomedullary cistern of conscious rats,enabling the study of the SpVc’s role in pain modulation.
作者
郭瑶
郭畅
王凯妮
臧若琪
高洁
马洋
陈怡彤
张宇
GUO Yao;GUO Chang;WANG Kaini;ZANG Ruoqi;GAO Jie;MA Yang;CHEN Yitong;ZHANG Yu(School and Hospital of Stomatology,Shanxi Medical University,Taiyuan 030001,China;Key Laboratory of Cellular Physiology,Ministry of Education,and the Department of Physiology,Shanxi Medical University,Taiyuan 030001,China)
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2024年第3期334-340,共7页
Chinese Journal of Neuroanatomy
关键词
三叉神经脊束核尾侧亚核
小脑延髓池
口面部疼痛
置管给药
神经炎症
大鼠
cerebellomedullary cistern(CMC)
caudal spinal trigeminal nucleus(SpVc)
oralfacial pain
catheter administration
neuroinflammation
rat
