摘要
目的观察完全弗氏佐剂(complete Freund’s adjuvant,CFA)诱导的口面部疼痛模型小鼠大脑前扣带回皮质(an⁃terior cingulate cortex,ACC)神经元电生理特性。方法将18只小鼠随机分为Sham组和CFA组,CFA组小鼠右侧面部胡须垫下注射CFA,构建口面部疼痛模型。Sham组小鼠注射生理盐水。用Von⁃Frey细丝检测口面部机械性疼痛阈值,采用膜片钳方法观察Sham组和CFA组小鼠ACC神经元兴奋性突触后电流(excitatory postsynaptic current,EPSC)和动作电位(action potential,AP)的发放情况。结果与Sham组相比,CFA组小鼠面部胡须垫下注射CFA后诱发机械性疼痛阈值较低(P<0.05)。CFA组小鼠ACC脑区神经元的EPSC的频率和幅值均高于Sham组(P<0.05)。当刺激电流在100、125、150、175、200 pA时,CFA组小鼠ACC脑区神经元的AP发放次数高于Sham组(P<0.05)。结论ACC脑区神经元活性在口面部疼痛的发生与发展中呈现增加的趋势,可能与口面部疼痛的形成密切相关。
Objective To observe the electrophysiological characteristics of anterior cingulate cortex(ACC)neurons in mice with orofacial pain induced by complete Freund’s adjuvant(CFA).Methods Eighteen mice were randomly divided into Sham group and CFA group.CFA was injected under the right facial beard pad in mice to construct a model of orofacial pain.Normal saline was injected in the Sham group.Von⁃Frey filament was used to detect the threshold of mechanical orofacial pain.The excitatory postsynaptic current(EPSC)and action potential(AP)of ACC neurons were observed in the Sham group and CFA group by patch clamp method.Results The threshold of mechanical pain in the CFA group was significantly lower than that in the Sham group after injection(P<0.05).The frequency and amplitude of the EPSC of ACC neurons in the CFA group were significantly higher than those in the Sham group(P<0.05).AP results showed that when the stimulus current was 100,125,150,175 and 200 pA,the discharge frequency of ACC neurons in the CFA group was significantly higher than that in the Sham group(P<0.05).Conclusion The activity of ACC neurons increases in the development and prgression of orofacial pain in mice and may be closely related to the formation of orofacial pain.
作者
郭梓琰
陈佳楠
董蕾
高天
张新海
Guo Ziyan;Chen Jianan;Dong Lei;Gao Tian;Zhang Xinhai(Department of Stomatology,The First Affiliated Hospital of Naval Medical University,Shanghai 200433,China)
出处
《海军医学杂志》
2024年第3期276-279,共4页
Journal of Navy Medicine
关键词
口面部痛
电生理
前扣带回皮质
兴奋性突触后电流
动作电位
Orofacial pain
Electrophysiology
Anterior cingulate cortex
Excitatory postsynaptic current
Action potential
