摘要
目的 观察温和灸对肝郁脾虚型腹泻型肠易激综合征(irritable bowel syndrome with predominant diarrhea, IBS-D)大鼠治疗效果及肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)的影响,探讨温和灸对肝郁脾虚型IBS-D大鼠的疗效及作用机制。方法 将24只Wistar雄性大鼠随机分为正常组、模型组、西药组和温和灸组,每组6只,西药组予匹维溴铵灌胃(15 mg/kg)口服,温和灸组选取双侧“天枢”“上巨虚”“太冲”进行温和灸治疗,正常组和模型组仅抓取、束缚,不做其他干预。观察各组大鼠一般情况;比较稀便率(LSR)、腹壁撤退反射(AWR)最小容量阈值、旷场实验结果;观察大鼠结肠组织病理形态学变化;检测大鼠血清和结肠组织中TNF-a和IL-10的含量。结果 (1)与正常组相比,模型组体质量和AWR最小容量阈值降低,LSR升高(P<0.05);与模型组相比,西药组、温和灸组体质量和AWR最小容量阈值增加,LSR减低(P<0.05);(2)与正常组相比,模型组总穿格数、直立次数、修饰次数减少(P<0.05);与模型组比较,西药组、温和灸组次数均增加(P<0.05);(3)与正常组相比,模型组血清和结肠组织中TNF-α含量升高,IL-10含量降低(P<0.01);与模型组比较,西药组、温和灸组血清和结肠组织中TNF-α含量降低,IL-10含量升高(P<0.05)。结论 温和灸治疗可缓解肝郁脾虚型IBS-D大鼠的腹痛、腹泻不适,改善其精神状态,其作用机制可能与调节TNF-α和IL-10水平,纠正炎症因子失调,增强免疫功能相关。
Objective To observe the therapeutic effect of mild moxibustion on rats with irritable bowel syndrome with predominant diarrhea(IBS-D) induced by liver-stagation and spleen-deficiency, and to detect the content of tumor necrosis factor-α(TNF-α)and interleukin-10(IL-10) in rats. Methods A total of 24 wistar male rats were randomly divided into a normal group, a model group, a western medicine group and a mild moxibustion group, with 6 rats in each group. Except the normal group, the other groups were treated with ice acetic acid enema + restraint stress to establish the IBS-D rat model with liver-depression and spleen-deficiency. After modeling, the western medicine group was given Piverium bromide intragastric administration(15 mg/kg), once a day. In the mild moxibustion group, acupuncture points of Tianshu, Shangjuxu and Taichong were selected for mild moxibustion treatment. The normal group and model group only grasp and bind, and do not do other intervention.After the intervention, the general condition of each group was observed. The rate of thin stool(LSR), the minimum volume threshold of the abdominal retract reflex(AWR) and the open field test were detected;the changes of colon histopathology were observed;and the conent of TNF-α and IL-10 in serum and colon tissues were detected. Results(1) Compared with the normal group, the body mass and the minimum volume threshold of AWR in the model group were both decreased while the LSR was increased(P<0.05). Compared with the model group, the body mass and the minimum volume threshold of AWR in the western medicine group and mild moxibustion group were increased, and LSR was decreased(P< 0.05).(2) Compared with the normal group, the total number of perforation, upright times and modification times in the model group were reduced(P<0.05);Compared with the model group, the times of western medicine group and mild moxibustion group were increased(P<0.05).(3) Compared with the normal group, the content of TNF-α in serum and colon tissue of model group was signific
作者
喻华梅
陆永军
杨孝芳
颜学军
韦祎
欧大芳
黄彦姬
朱洲
张思露
YU Huamei;LU Yongjun;YANG Xiaofang;YAN Xuejun;WEI Yi;OU Dafang;HUANG Yanji;ZHU Zhou;ZHANG Silu(Guizhou University of Traditional Chinese medicine,Guiyang 550025,Guizhou,China;Du Yun Municipal People's Hospital,Duyun 558000,Guizhou,China)
出处
《贵州中医药大学学报》
2024年第4期15-21,共7页
Journal of Guizhou University of Traditional Chinese Medicine
基金
贵州省中医药管理局中医药、民族医药科学技术研究专项课题(QZYY-2023-137)。
关键词
温和灸
肝郁脾虚
腹泻型肠易激综合征
肿瘤坏死因子-α
白细胞介素-10
mild moxibustion
liver-stagnationn and spleen-deficiency type
irritable bowel syndrome with predominant diarrhea
tumor necrosis factor-α
interleukin-10
