摘要
采用化学还原法制备了氧化石墨烯-金纳米球(GO-AuNP),利用透射电子显微镜(TEM)、紫外-分光光度计(UV-Vis)和激光粒度仪等对GO-AuNP进行了表征,并利用红外热成像仪对其光热性能进行了研究。将GO-AuNP负载抗癌药物盐酸阿霉素(DOX)制备成纳米药物复合物(GO-AuNP@DOX),并通过荧光分光光度计对DOX的负载和释放进行了检测;结果显示,GO-AuNP@DOX中DOX的释放在弱酸性环境下更优,并且在808 nm激光照射条件下pH=5.3时释放量可以达到30.51%。采用共聚焦显微镜分析了癌细胞对GO-AuNP@DOX的摄取能力;采用CCK-8细胞毒性实验分析了GO-AuNP@DOX的体外杀伤肿瘤细胞能力,细胞毒性实验证明GO-AuNP载体具备良好的生物相容性,体内抗肿瘤实验结果表明,荷瘤小鼠在化疗光热协同作用可以很好地抑制肿瘤生长。结果表明,GO-AuNP纳米药物载体具有光热转换能力优异、生物相容性优良的优点,其pH/近红外光谱(NIR)双重药物控释性能使药物载体在化疗-光热协同治疗肿瘤方面具有潜在的应用价值。
The present study employed a chemical reduction method to synthesize gold nanoparticles(AuNPs)decorated on graphene oxide(GO)nanomaterials.The produced GO-AuNPs were characterized using advanced analytical techniques such as transmission electron microscopy,UV-visible spectrophotometry,and laser particle size analysis.Furthermore,the photothermal properties of GO-AuNPs were investigated using an infrared thermal imaging camera.Subsequently,the GO-AuNPs were utilized for the loading of an anti-cancer drug,doxorubicin hydrochloride(DOX),to yield a nanodrug complex(GO-AuNP@DOX).The loading and release of DOX were determined by fluorescence spectrophotometry,revealing that the DOX release was more favorable under weak acidic conditions.Moreover,it was observed that at pH=5.3,the release amount of DOX reached up to 30.51%under the irradiation of 808 nm laser.The cellular uptake ability of GO-AuNP@DOX by cancer cells was analyzed using confocal microscopy.The in vitro cytotoxicity of GO-AuNP@DOX against tumor cells was evaluated using a CCK-8 cell viability assay,demonstrating the excellent biocompatibility of the GO-AuNP carrier.Additionally,in vivo anti-tumor experiments conducted on tumor-bearing mice revealed that the combination of chemotherapy and photothermal therapy effectively suppressed tumor growth.These results indicate that the GO-AuNP nanodrug carrier possesses outstanding photothermal conversion ability and exceptional biocompatibility,while their pH/NIR dual drug release performance makes it a promising candidate for synergistic chemo-photothermal therapy of tumors.
作者
郭国英
赵墨晅
梁文婷
弓韬
董川
GUO Guo-Ying;ZHAO Mo-Xuan;LIANG Wen-Ting;GONG Tao;DONG Chuan(Department of Biochemistry and Molecular Biology,Shanxi Medical University,Taiyuan 030001,China;Institute of Environmental Science,Shanxi University,Taiyuan 030006,China)
出处
《应用化学》
CAS
CSCD
北大核心
2024年第7期976-986,共11页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(No.21976113)
山西省自然科学基金(No.202103021224240)资助。
关键词
金纳米球
氧化石墨烯
药物载体
抗癌
Gold nanospheres
Graphene oxide
Drug carrier
Anti-cancer
