摘要
建立了高效液相色谱-串联质谱检测人参配方颗粒中18种人参皂苷定性定量的分析方法。样品经80%甲醇超声提取,采用Agilent Poroshell 120 EC-C_(18)色谱柱(2.1 mm×150 mm,2.7μm)分离,以0.1%甲酸乙腈和0.1%甲酸水溶液作为流动相进行梯度洗脱,电喷雾负离子模式,进行预设定多反应监测(sMRM)模式检测。结果显示,18种目标分析物在各自线性范围内线性良好,相关系数均大于0.9970。平均加标回收率为94.5%~105.2%,RSD为1.6%~3.9%。采用本方法对12份样品进行测定,发现4份样品中检测出拟人参皂苷成分。所建方法快速、可靠、灵敏,适用于人参配方颗粒中18种人参皂苷的定性及定量分析。
To develop a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for qualitative and quantitative analysis of 18 ginsenosides in ginseng formulation granules.Samples were extracted by 80%methanol with ultrasound extraction.The ginsenosides were separated on an Agilent Poroshell 120 EC-C18 column(2.1 mm×150 mm,2.7μm)by gradient elution with 0.1%formic acid acetonitril and 0.1%formic acid water solution as mobile phase.A scheduled multiple reaction monitoring(sMRM)in negative ion mode was adopted in mass spectrometry acquisition.As a result,the linear correlation coefficients(r)of the 18 analytes in the linear ranges were more than 0.9970.The mean recoveries were all in the range of 94.5%~105.2%,and the relative standard deviations(RSD)were between 1.6%and 3.9%.Pse F11 was detected in 4 batches(12 batches in total)samples.The method was proved to be rapid,reliable and sensitive,which can be used to qualitative and quantitative analysis of 18 ginsenosides in ginseng formulation granules.
作者
庄玥
黄艳婷
韩毓之
刘亚雄
邱蕴绮
Zhuang Yue;Huang Yanting;Han Yuzhi;Liu Yaxiong;Qiu Yunqi(Guangdong University of Technology,Guangzhou 510006,China;Guangdong Institute for Drug Control,Guangzhou 510663,China)
出处
《广东化工》
CAS
2024年第9期152-155,共4页
Guangdong Chemical Industry
基金
广东省医学科学技术研究基金项目(B2021409)
广东省中医药局科研项目(20221055)。
