摘要
[目的]比较不同产地黄芪中黄芪甲苷含量的大小。[方法]采用高效液相-蒸发光散射检测法(HPLC-ELSD)测定黄芪中黄芪甲苷的含量,色谱柱为Agilent C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-水(40∶60);流速为1.0 mL/min;柱温为30℃;进样量为5μL。ELSD检测器漂移管温度为100℃;载气压力为200 kPa。[结果]不同产地黄芪中黄芪甲苷含量分别为甘南0.147%、陇西0.117%、岷县0.205%、山西0.182%、张掖0.067%、武威0.045%、庆阳0.097%、渭源0.114%、内蒙古0.087%、新疆0.046%。[结论]不同产地黄芪中黄芪甲苷的含量差异较大,4个黄芪主产省(自治区)中甘肃产的黄芪中黄芪甲苷含量最高,山西、陕西产的黄芪中黄芪甲苷含量相对较高,新疆产的黄芪中黄芪甲苷含量较低。同一省份中陇西、甘南、岷县、庆阳、渭源县产黄芪中黄芪甲苷的含量较高,而武威和张掖产黄芪中黄芪甲苷的含量偏低。
[Objective]To determine the content of astragaloside in Astragalus membranaceus from different habitats.[Method]HPLC-ELSD was used to determine the content of astragaloside.The analysis was performed on Agilent C_(18)(4.6 mm×250 mm,5μm)column with the mixture of acetonitrile-water(40∶60)as mobile phase,and the flow rate was 1.0 mL/min;the column temperature was 30℃;the temperature of drift tube in ELSD was 100℃and the gas pressure was 200 kPa.[Result]The astragaloside contents in Astragalus membranaceus from Gannan,Longxi,Minxian,Shanxi,Zhangye,Wuwei,Qingyang,Weiyuan,Inner Mongolia and Xinjiang were 0.114%,0.117%,0.205%,0.182%,0.067%,0.045%,0.097%,0.114%,0.087%and 0.046%.[Conclusion]The content of astragaloside in Astragalus membranaceus from different production areas is quite different.Among them,the content of astragaloside in Gansu is the highest,the content of astragaloside in Astragalus membranaceus produced in Shanxi and Shaanxi is relatively higher,and the content of astragaloside in Astragalus membranaceus produced in Xinjiang is lower.In the same province,the content of astragaloside in Astragalus membranaceus in Longxi,Gannan,Minxian,Qingyang and Weiyuan counties was higher,while the content of astragaloside in Wuwei and Zhangye was low.
作者
邹传宗
吴秋钰
ZOU Chuan-zong;WU Qiu-yu(Department of Pharmacy,Gansu Medical College,Pingliang,Gansu 744000)
出处
《安徽农业科学》
CAS
2022年第1期208-209,227,共3页
Journal of Anhui Agricultural Sciences
基金
甘肃省教育厅项目(2019B-199)。
关键词
黄芪
黄芪甲苷
HPLC
含量测定
Astragalus membranaceus
Astragaloside
HPLC
Content determination
