摘要
采用菌落直径法测定茶树炭疽病菌山茶刺盘孢(Colletotrichum camelliae)对10种化学杀菌剂的敏感性,以期为该病原引起的茶炭疽病的田间防控提供参考依据。结合试验数据分析表明,75%百菌清和50%甲基硫菌灵在5μg/mL时抑菌率就达到了100%;10%苯醚甲环唑和250 g/L丙环唑在50μg/mL时抑菌率分别达到了92.88%,100%;250 g/L吡唑醚菌酯、40%腈菌唑、80%代森锰锌、250 g/L嘧菌酯、25 g/L咯菌腈和80%代森锌的抑菌效果依次减弱。并对10种化学杀菌剂的抑菌率进行分析,分别计算出毒力回归方程、相关系数及半数有效浓度(EC_(50))。研究发现50%甲基硫菌灵、75%百菌清、250 g/L丙环唑和10%苯醚甲环唑对茶树炭疽菌有较强的毒力。
The high efficient chemical fungicides against tea anthracnose(Colletotrichum camelliae)were screened to provide a reference and basis for the control of tea anthracnose caused by this pathogen in the field.The susceptibility of the pathogen to 10 chemical fungicides was determined by colony diameter method.Comprehensive analysis of the experimental data reflects that the inhibition rates of 75%chlorothalonil and 50%thiophanate-methyl reached 100%at a concentration of 5μg/mL.At 50μg/mL,the inhibition rates of 10%phenyl ether metronidazole and 250 g/L propiconazole reached 92.88%and 100%respectively.The inhibition effects of 250 g/L pyraclostrobin,40%cyproconazole,80%diclofenac sodium,250 g/L pyrimethamine,25 g/L fumonisin and 80%diclofenac sodium gradually weakened.The inhibition rates of 10 chemical fungicides were also analyzed and the toxicity regression equations,correlation coefficients and EC50 values were calculated respectively.It was found that 75%chlorothalonil,250 g/L propiconazole,50%thiophanatemethyl and 10%phenyl ether metronidazole have strong toxicity against tea anthracnose.
作者
张景贺
张瑾
孙晓玲
王占斌
ZHANG Jinghe;ZHANG Jin;SUN Xiaoling;WANG Zhanbin(Northeast Forestry University,Haerbin 150040,China;Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008,China)
出处
《中国茶叶》
2024年第8期35-39,共5页
China Tea
基金
海南省重点研发计划(ZDYF2021XDNY274)。
关键词
茶炭疽病
毒力测定
化学杀菌剂
tea anthracnose
virulence assay
chemical fungicide