摘要
目的探讨下肢动脉硬化闭塞症(LEASO)患者支架植入术后血清长链非编码RNA(LncRNA)-肺腺癌转移相关转录本1(MALAT1)、LncRNA-X失活特异性转录本(XIST)与1年内支架内再狭窄(ISR)的相关性。方法回顾性选取2018年1月—2022年12月在内蒙古医科大学附属肿瘤医院血管外科行支架植入术的LEASO患者147例作为研究对象,根据1年内是否发生ISR分为ISR组41例和无ISR组106例,采用实时荧光定量PCR检测术后血清LncRNA-MALAT1、LncRNA-XIST水平;通过Pearson法分析LEASO患者血清LncRNA-MALAT1、LncRNA-XIST水平与管腔直径缩小率的相关性;多因素Logistic回归分析LEASO患者支架植入术后1年内ISR的影响因素;ROC曲线分析血清LncRNA-MALAT1、LncRNA-XIST水平对LEASO患者支架植入术后1年内ISR的预测价值。结果随访1年,147例LEASO患者支架植入术后ISR发生率为27.89%(41/147);与无ISR组比较,ISR组血清LncRNA-MALAT1、LncRNA-XIST水平升高(t/P=5.949/<0.001、5.927/<0.001);Pearson相关性分析显示,LEASO患者血清LncRNA-MALAT1、LncRNA-XIST水平与管腔直径缩小率呈正相关(r/P=0.596/<0.001、0.588/<0.001);多因素Logistic回归显示,糖尿病和C反应蛋白、LncRNA-MALAT1、LncRNA-XIST升高为LEASO患者支架植入术后1年内ISR的独立危险因素[OR(95%CI)=3.185(1.155~8.781)、1.187(1.003~1.404)、1.433(1.199~1.713)、1.575(1.242~1.998)];ROC曲线显示,血清LncRNA-MALAT1、LncRNA-XIST水平联合预测LEASO患者支架植入术后1年内ISR的曲线下面积为0.906,大于血清LncRNA-MALAT1、LncRNA-XIST水平单独预测的0.799、0.786(Z/P=3.176/0.002、3.513/<0.001)。结论LEASO患者支架植入术后血清LncRNA-MALAT1、LncRNA-XIST水平升高是1年内ISR的独立危险因素,血清LncRNA-MALAT1、LncRNA-XIST水平联合对LEASO患者支架植入术后1年内ISR有较高的预测价值。
Objective To investigate the correlation between serum long-chain non-coding RNA(LncRNA)-metastasis-adenocarcinoma metastasis-associated transcript 1(MALAT1),LncRNA-X inactivation specific transcript(XIST)and in-stent restenosis(ISR)within 1 year after stenting in patients with lower extremity arteriosclerosis obliterans(LEASO).Methods One hundred and forty-seven LEASO patients who underwent stent implantation in the Department of Vascular Surgery of the Affiliated Cancer Hospital of Inner Mongolia Medical University from January 2018 to December 2022 were selected as the study subjects,and they were divided into the ISR group(41 cases)and the no-ISR group(106 cases)based on the occurrence of ISR within 1 year,and the real-time fluorescence quantitative PCR was used to detect the postoperative serum LncRNA-MALAT1,LncRNA-XIST levels;the correlation between serum LncRNA-MALAT1 and LncRNA-XIST levels and lumen diameter reduction rate in LEASO patients was analyzed by Pearson's method,the factors of ISR within 1 year after stent implantation in LEASO patients were analyzed by multifactorial logistic regression,and ROC curves were plotted to analyze the serum LncRNA-MALAT1,The predictive value of LncRNA-XIST levels for ISR within 1 year after stenting in LEASO patients.Results At 1-year follow-up,the incidence of ISR after stenting was 27.89%(41/147)in 147 LEASO patients;serum LncRNA-MALAT1 and LncRNA-XIST levels were elevated in the ISR group compared with the no-ISR group(t/P=5.949/<0.001,5.927/<0.001);Pearson's correlation showed a positive correlation between serum LncRNA-MALAT1 and LncRNA-XIST levels and the rate of lumen diameter reduction in LEASO patients(r/P=0.596/<0.001,0.588/<0.001);Multifactorial logistic regression showed that diabetes mellitus and elevated C-reactive protein,LncRNA-MALAT1,and LncRNA-XIST were independent risk factors for ISR within 1 year of stent implantation in patients with LEASO[OR(95%CI)=3.185(1.155-8.781),1.187(1.003-1.404),1.433(1.199-1.713),1.575(1.242-1.998)];The ROC curve showe
作者
那顺孟和
玉海
陈恕
王磊
姚顺
李巍
云昕裔
Nashun Menghe;Yu Hai;Chen Shu;Wang Lei;Yao Shun;Li Wei;Yun Xinyi(Department of Vascular Surgery,Peking University Cancer Hospital Inner Mongolia Hospital,Huhhot 010020,China;不详)
出处
《疑难病杂志》
CAS
2024年第8期913-918,共6页
Chinese Journal of Difficult and Complicated Cases
基金
内蒙古自治区自然科学基金(2019MS06134)。
