摘要
Dirigent(DIR)在植物木脂素或木质素的产生、防御反应、次生代谢和抗病性中起重要作用。为探究谷子DIR家族基因(SiDIRs)的生物学功能,本研究对其进行生物信息学和盐碱胁迫下的基因表达分析。结果表明,共鉴定到41个SiDIRs,不均匀分布在8条染色体上,根据其在染色体上的位置将其命名为SiDIR1~SiDIR41,根据系统进化树关系将其划分为5个亚家族。共线性分析结果表明,SiDIRs同单子叶植物高粱、玉米和水稻的共线性关系远高于双子叶植物拟南芥、番茄和大豆。顺势作用元件分析结果表明,大多数SiDIRs具有与激素、植物生长、环境胁迫和光相关的元件。利用String软件预测到7个SiDIRs蛋白质的相互作用网络。SiDIRs在根、茎、叶和花中具有组织表达特异性,表明它们在植物生长发育中发挥不同的作用。分析盐碱胁迫下12个SiDIRs基因的表达水平发现,B175(盐碱敏感)和JK3(耐盐碱)较对照均有不同程度的变化,SiDIR17和SiDIR28在JK3中呈上调表达,但在B175中呈下调表达,推测这两个基因可能参与谷子耐盐碱胁迫。本研究结果可为深入解析SiDIRs的耐盐碱分子机制提供理论基础,为今后培育高效耐盐碱谷子新品种提供基因资源。
Dirigent(DIR)plays important roles in plant lignan or lignin production,defense response,secondary metabolism and disease resistance.In order to explore the biological function of DIR family genes(SiDIRs)in foxtail millet,bioinformatics and gene expression analysis under saline-alkali stress were carried out in this study.As a result,41 SiDIRs were identified,which were unevenly distributed on eight chromosomes and named as SiDIR1-SiDIR41 according to their chromosomal locations.According to the phylogenetic tree,SiDIRs were divided into five subfamilies.The result of collinearity analysis revealed that SiDIRs had higher collinearity with the genes in monocotyledonous plants than those in dicotyledonous plants.The analysis of cis-acting elements indicated that most SiDIRs possess cis-acting elements related to hormones,plant growth,stress response,and light response.The interaction network of seven SiDIRs proteins was predicted by String software.SiDIRs exhibited tissue-specific expression in roots,stems,leaves,and flowers,indicating that they play different roles in plant growth and development.The analysis of the expression levels of 12 SiDIRs genes under saline-alkaline stress showed that B175(saline-alkaline sensitive)and JK3(saline-alkaline tolerant)had different expression levels of changes compared with the control.SiDIR17 and SiDIR28 showed an upward trend in JK3 but a downward trend in B175,suggesting that these two genes may be involved in the saline-alkaline tolerance of foxtail millet.The results of this study provide a theoretical basis for in-depth analysis of the molecular mechanism underlying saline-alkali tolerance of SiDIRs,as well as important genetic resources for the cultivation of new millet varieties with high-efficiency saline-alkali tolerance in the future.
作者
赵玉雪
任学军
李云
王慧敏
周小可
林小虎
韩玉翠
ZHAO Yuxue;REN Xuejun;LI Yun;WANG Huimin;ZHOU Xiaoke;LIN Xiaohu;HAN Yucui(College of Agronomy and Biotechnology,Hebei Normal University of Science and Technology/Hebei Key Laboratory of Crop Stress Biology,Qinhuangdao,Hebei 066004;Analysis and Testing Center,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004;Research Center of Rural Vitalization,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004)
出处
《核农学报》
CAS
CSCD
北大核心
2024年第9期1647-1659,I0001,共14页
Journal of Nuclear Agricultural Sciences
基金
河北省现代农业产业技术体系创新团队(杂粮杂豆)项目(HBCT2023050404)
秦皇岛市科学技术研究与发展计划(202201B023)
河北科技师范学院科学研究基金(2021YB007)。
