摘要
为探究不同产地马蹄金的挥发性成分,建立气相色谱-串联质谱法对河北、广西、四川、贵州、浙江5个产地的马蹄金样品挥发性成分进行测定,采用面积归一化法计算各成分的相对含量。称取0.5 g马蹄金样品,以乙酸乙酯为提取溶剂,冷浸2 h,低温超声提取30 min,滤液作为样品溶液。通过谱库检索,5个产地马蹄金的挥发性成分中共鉴定出有机酸、醇、烯、酯、烷烃等35种化合物,其中14种是共有成分,6种是产地特有成分,相对含量较高的挥发性成分分别为十六烷酸、亚油酸、桦木醇、α-亚麻酸、β-谷甾醇、菜油甾醇、豆甾醇,相对含量分别为27.99%、13.14%、12.91%、11.83%、11.64%、8.16%、5.28%。该方法可对马蹄金中多种挥发性成分进行定量分析,为马蹄金的质量控制和药效研究提供参考。
To explore the constituents of volatile components in dichondra from different producing areas,gas chromatography-mass spectrometry was used to test the volatile components of dichondra samples from Hebei,Guangxi,Sichuan,Guizhou,and Zhejiang Province,and the relative contents of the components were calculated using peak area normalization method.0.5 g of Dichondra repens sample was weighed,and ethyl acetate was used as the extraction solvent,cold dipped for 2 h,low temperature ultrasonic extraction for 30 min,and the filtrate was used as the sample solution.Thirty-five components such as organic acid,alcohols,alkenes,esters and alkanes from five areas of dichondra were illustrated by library searching.Among them,fourteen kinds were common components,and another six kinds were the specific components of the area.The hexadecyclic acid,linoleic acid,betulinol,α-linolenic acid,β-sitosterol,canola oil sterol and stigasterol were the relatively high content of volatile components,the relative contents were 27.99%,13.14%,12.91%,11.83%,11.64%,8.16%and 5.28%.This method can be used for quantitative analysis of various volatile components in the dichondra,which provides a reference for the quality control and pharmacodynamic study of the gold.
作者
郭海平
杨红梅
马丽
周维维
王志轩
王世权
董建锋
GUO Haiping;YANG Hongmei;MA Li;ZHOU Weiwei;WANG Zhixuan;Wang Shiquan;Dong Jianfeng(Chengde Food and Drug Inspection Institute,Yanshan genuine medicinal materials in Hebei Province Inspection and Test technology innovation Center,Chengde 067000,China;Chengde Nursing Vocational College,Chengde 067000,China)
出处
《化学分析计量》
CAS
2024年第7期79-84,共6页
Chemical Analysis And Meterage
关键词
气相色谱-串联质谱
马蹄金
挥发性成分
中药产地
gas chromatography-mass spectrometry
dichondra
volatile components
origin of Chinese medicine
