摘要
目的对比黄芪饮片的性状、气味、指纹图谱、灭菌效果及浸出物、有效成分含量,以考察不同灭菌方式对黄芪饮片质量的影响,进一步优选黄芪饮片的最佳灭菌工艺参数。方法对比湿热灭菌、辐照灭菌、干热灭菌对黄芪饮片质量的影响,选择最佳灭菌方式。基于此,以黄芪甲苷含量、毛蕊异黄酮葡萄糖苷含量、两者总含量和灭菌率为考察指标,灭菌温度、灭菌时间、物料厚度为考察因素,在单因素试验的基础上,采用响应面法优化黄芪饮片最佳干热灭菌工艺。结果选择干热灭菌方式对黄芪饮片进行灭菌;干热灭菌最佳工艺为:灭菌温度113℃、灭菌时间5.1 h、物料厚度19 mm。结论综合考虑灭菌效果、对有效成分含量的影响、操作性及经济成本,选择干热灭菌为黄芪饮片的最佳灭菌方式,优选得到的灭菌工艺稳定可行、重复性好,可为其工业化生产提供科学依据。
Objective To investigate the effects of different sterilization methods on the quality of Astragali Radix decoction pieces by comparing the properties,odor,fingerprint,sterilization effect,extract and active component contents of Astragali Radix decoction pieces,and to further optimize the best sterilization process parameters of Astragali Radix decoction pieces.Methods The effects of moist heat sterilization,irradiation sterilization and dry heat sterilization on the quality of Astragali Radix decoction pieces were compared,and the best sterilization method was selected.Based on this,taking the content of astragaloside IV,the content of isoflavone glucoside,the total content of the two and the sterilization rate as the assessment indicators,taking the sterilization temperature,sterilization time and material thickness as the assessment factors,on the basis of the single-factor test,the response surface method was used to optimize the optimal dry heat sterilization process of Astragali Radix decoction pieces.Results The dry heat sterilization method was selected to sterilize Astragali Radix decoction pieces.The best process for dry heat sterilization was as follows:the sterilization temperature of 113°C,sterilization time of 5.1 h,and the material thickness of 19 mm.Conclusion Considering the sterilization effect,the influence on the content of active ingredients,operability and cost,selecting dry heat sterilization is the best sterilization method for Astragali Radix decoction pieces,and the optimal sterilization process is stable and feasible,with good repeatability,which can provide scientific basis for its industrial production application.
作者
高艳红
曹玲
崔琳琳
金宗元
周冰
李晨鹏
赵利枝
GAO Yanhong;CAO Ling;CUI Linlin;JIN Zongyuan;ZHOU Bing;LI Chenpeng;ZHAO Lizhi(Lunan Pharmaceutical Group Co.,Ltd.,Linyi 276006,Shandong Province,China;National Key Laboratory for The Integration and Innovation of Classical Prescriptions and Modern Traditional Chinese Medicine,Linyi 276006,Shandong Province,China;Chinese Medicine Generic Technology Shandong Engineering Research Center,Linyi 276006,Shandong Province,China)
出处
《中国药师》
CAS
2024年第7期1134-1141,共8页
China Pharmacist
基金
山东省重点研发计划(重大科技创新工程)项目(2021CXGC010508)
山东省新旧动能转换重大产业攻关项目(鲁动能办[2021]23号)。
关键词
黄芪
湿热灭菌
干热灭菌
辐照灭菌
层次分析法
响应面
Astragalus
Moist heat sterilization
Dry heat sterilization
Irradiation sterilization
Analytic hierarchy process
Response surface
