摘要
目的:基于网络药理学探讨诃子治疗慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)的作用机制。方法:通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)检索诃子的化学成分,Swiss Target Prediction数据库获取诃子的作用靶点。采用OMIM、GeneCards、DisGeNET数据库预测COPD作用靶点,借助微生信平台获取诃子与COPD的交集靶点。将交集靶点导入STRING数据库,构建蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络。基于Centispace插件进行拓扑分析,从而筛选诃子治疗COPD的核心靶点。采用DAVID数据库进行基因本体(Gene Ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析。结果:诃子活性成分8种,作用靶点333个,活性成分包括鞣花酸、番泻苷E、7-脱氢豆甾醇等。COPD作用靶点8805个,其与诃子的交集靶点253个。通过筛选得到10个核心靶点,包括信号转导和转录激活因子3(signal transducer and activator of transcription 3,STAT3)、丝氨酸/苏氨酸蛋白激酶1(serine/threonine kinase 1,AKT1)、丝裂原活化蛋白激酶3(mitogen-activated protein kinase 3,MAPK3)等。GO功能分析主要富集于细胞凋亡、G蛋白偶联血清素受体活性、RNA聚合酶Ⅱ转录因子活性等,KEGG通路主要包括PI3K-Akt信号通路、HIF-1信号通路、ErbB信号通路等。结论:诃子通过多成分、多通路、多靶点发挥治疗COPD的作用。
Objective:To study on the mechanism of Hezi(Chebulae Fructus)in treating chronic obstructive pulmonary disease(COPD)based on network pharmacology.Methods:The chemical ingredients of Hezi were retrieved through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and the target points of Hezi were obtained from the Swiss Target Prediction database.The targets of COPD were predicted by using OMIM,GeneCards,and DisGeNET databases.The intersection targets of Hezi and COPD were obtained by using the microbiome plaform.A protein-protein interaction(PPI)network was constructed by importing the intersection targets into the STRING database.A topology analysis was performed based on the Centispace plugin to screen the core targets of Hezi for treating COPD.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KECG)analysis was performed by using the DAVID database.Results:There were 8 active ingredients and 333 targets of action in Hezi,including tannic acid,sennoside E,7-dehydrogustosterol,etc.There were 8,805 targets for COPD,and 253 targets for its intersection with Hezi.Ten core targets were identified through screening,including signal transducer and activator of transcription 3(STAT3),serine/threonine kinase 1(AKT1),and mitogen activated protein kinase 3(MAPK3).CO functional analysis is mainly enriched in cell apoptosis,G-protein coupled serotonin receptor activity,RNA polymerase II transcription factor activity,etc.The KEGG pathway mainly included the PI3K Akt signaling pathway,HIF-1 signaling pathway,and ErbB signaling pathway.Conclusion:Hezi exerts therapeutic effects on COPD through multiple ingredients,pathways,and targets.
作者
陈佳瑞
徐新毅
梅霄
金朝阳
CHEN Jiarui;XU Xinyi;MEI Xiao;JIN Chaoyang(Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou,China,550025;The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou,China,550003)
出处
《河南中医》
2024年第8期1224-1229,共6页
Henan Traditional Chinese Medicine
关键词
诃子
慢性阻塞性肺疾病
网络药理学
鞣花酸
Hezi(Chebulae Fructus)
chronic obstructive pulmonary disease(COPD)
network pharmacology
tannic acid
