摘要
目的:研究“孕产妇复合营养粉”对斑马鱼生长性能和氨基酸含量的影响。方法:选取野生型2月龄AB品系斑马鱼,设置5个处理组(复合营养粉浓度为1.0 mg·mL^(-1)、2.0 mg·mL^(-1)、4.0 mg·mL^(-1)、8.0 mg·mL^(-1)、16.0 mg·mL^(-1))及阴性组,每日灌胃2次,确定最大耐受浓度(Maximum Tolerated Concentration,MTC);设计3个浓度梯度组(1/4 MTC、1/2 MTC和MTC)、阳性组及阴性组,利用斑马鱼模型,从增重率、特定生长率、氨基酸含量变化等方面对比分析数据。结果:复合营养粉对正常斑马鱼最大耐受浓度为1.0 mg·mL^(-1);浓度为0.50 mg·mL^(-1)和1.00 mg·mL^(-1)时可显著提高2月龄斑马鱼增重率、特定生长率。饲料添加复合营养粉能显著提升斑马鱼总氨基酸和必需氨基酸含量。结论:复合营养粉对斑马鱼的生长性能及氨基酸含量具有明显的补充改善作用。
Objective:To study the effects of“maternal compound nutrition powder”on growth performance and amino acid content of zebrafish.Method:Wild type 2-month-old AB strain zebrafish were selected and divided into 5 treatment groups(complex nutrient powder concentration were 1.0 mg·mL^(-1),2.0 mg·mL^(-1),4.0 mg·mL^(-1),8.0 mg·mL^(-1),16.0 mg·mL^(-1))and negative group were administered twice a day to determine the maximum tolerated concentration(MTC).Three concentration gradient groups(1/4 MTC,1/2 MTC and MTC),positive group and negative group were designed,and the data in terms of weight gain rate,specific growth rate and amino acid content were compared by using zebrafish model.Result:The maximum tolerance concentration of compound nutrition powder to normal zebrafish was 1.0 mg·mL^(-1).When the concentrations were 0.50 mg·mL^(-1) and 1.00 mg·mL^(-1),the weight gain rate and specific growth rate of 2-month-old zebrafish were significantly increased.The contents of total amino acid and essential amino acid of zebrafish were significantly increased by adding compound nutrition powder.Conclusion:The compound nutrition powder can significantly improve the growth performance and amino acid content of zebrafish.
作者
李桢
李英
李欣
段梦妮
岳林涛
LI Zhen;LI Ying;LI Xin;DUAN Mengni;YUE Lintao(Shanxi Key Laboratory of Homologous Functional Food for Medicine and Food,Changzhi 047100,China;Shanxi Zhendong Pharmaceutical Co.,Ltd.,Changzhi 047100,China;Shanxi Zhendong Wuhe Hospital Co.,Ltd.,Changzhi 047100,China)
出处
《食品安全导刊》
2024年第14期77-80,共4页
China Food Safety Magazine
关键词
斑马鱼
复合营养粉
生长性能
氨基酸含量
zebrafish
compound nutrition powder
growth performance
amino acid content
