摘要
目的:基于肠道菌群研究不同产地的苍术的抗腹泻作用,为苍术的道地性研究提供依据。方法:采用大黄水煎液复制小鼠腹泻模型,给予不同产地的苍术水煎液灌胃7 d,观察小鼠状态,检测粪便评分和含水量、小肠推进率、结肠病理变化以及水通道蛋白AQP3和AQP4表达;利用16S rRNA测序检测小鼠肠道菌群。结果:与模型组相比,道地苍术与非道地苍术均可以改善腹泻小鼠表型指标,且道地苍术(江苏产地)对腹泻小鼠的小肠推进抑制率优于非道地苍术(湖北产地),增加水通道蛋白AQP3和AQP4的能力优于非道地苍术(河北产地和湖北产地)。16S rRNA测序结果显示,道地苍术与非道地苍术均可改善腹泻小鼠肠道菌群beta多样性。在门水平上,道地苍术(江苏产地)和非道地苍术(河北产地和湖北产地)均可降低厚壁菌门(Firmicutes)丰度和增加拟杆菌门(Bacteroidota)丰度,不同的是,道地苍术(江苏产地)可以降低放线菌门(Actinobacteriota)的丰度,增加疣微菌门(Verrucomicrobia)的丰度;非道地苍术(河北产地)增加疣微菌门(Verrucomicrobia)的丰度。在属水平上,相对于非道地苍术(河北产地和湖北产地),道地苍术(江苏产地)调控更多的微生物数量。结论:道地苍术抗腹泻作用以及增加水通道蛋白AQP3和AQP4表达的能力优于非道地苍术,这些效应与道地苍术对肠道菌群种类和数量调节有关。
Objective:The anti-diarrhea effect of Atractylodis Rhizome from different areas was studied based on the gut microbiota,which provides the basis for the geoherbalism research of Atractylodis Rhizome.Methods:The diarrhea model was induced by rhubarb aqueous extract(10 g/kg).Then mice were treated with Atractylodis Rhizome aqueous extract(1.85 g/kg)from different areas for 7 days.The status of the mice was observed,and fecal score,fecal water content,small intestinal propulsion rate,and pathological changes and the expression of AQP3 and AQP4 in the colon.The changes of gut microbiota in mice were observed by 16S rRNA sequencing.Results:Compared with the model group,both Atractylodis Rhizome from genuine producing areas and non-genuine producing areas could significantly improve the phenotypic indicators of diarrhea mice.Furthermore,Atractylodis Rhizome from genuine producing areas(Jiangsu)has a better inhibitory rate on small intestinal propulsion in mice with diarrhea than Atractylodis Rhizome from non-genuine producing areas(Hubei),and its ability to increase AQP3 and AQP4 is better than Atractylodis Rhizome from non-genuine producing areas(Hebei and Hubei).In addition,16S rRNA sequencing analysis showed Atractylodis Rhizome from genuine producing areas and non-genuine producing areas could improve beta diversity of gut microbiota.Species composition of gut microbiota showed that Atractylodis Rhizome from genuine producing areas(Jiangsu)and non-genuine producing areas(Hebei and Hubei)reduced the abundance of Firmicutes while increased the abundance of Bacteroidota.The distinction is that Atractylodis Rhizome from genuine producing areas(Jiangsu)can diminish Actinobacteriota abundance while increasing Verrucomicrobia;Atractylodis Rhizome from non-genuine producing areas(Hubei)increased Verrucomicrobia.At the genus level,Atractylodis Rhizome from genuine producing areas(Jiangsu)and non-genuine producing areas(Hebei and Hubei)regulated distinct species,with genuine producing areas(Jiangsu)having the most regulated
作者
刘娟
张丹丹
程浩
周耀钏
吴金露
冯五文
彭成
Liu Juan;Zhang Dandan;Cheng Hao;Zhou Yaochuan;Wu Jinlu;Feng Wuwen;Peng Cheng(Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu,610072,China;State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China)
出处
《成都中医药大学学报》
2024年第4期1-8,共8页
Journal of Chengdu University of Traditional Chinese Medicine
基金
国家中医药管理局西南特色中药资源多维评价多学科交叉创新团队项目(ZYYCXTD-D-202209)。
关键词
苍术
道地性
腹泻
水通道蛋白
肠道菌群
Atractylodis Rhizome
Geoherbalism
Diarrhea
Aquaporin
Gut microbiota
