摘要
目的探讨活血荣络方对缺氧缺血性脑损伤(HIBD)大鼠的保护作用, 明确HIBD损伤及修复过程中AMPK信号通路的表达情况。方法在缺氧缺血再灌注模型基础上采用改良缺氧瓶建立足月HIBD大鼠模型。将造模成功60只大鼠按随机数字表法分为模型组和中药组, 每组30只, 另设对照组30只。术前2 h中药组灌胃活血荣络方水煎液1.17 g/100 g(等效剂量), 对照组和模型组灌胃等体积生理盐水, 术后2 h再灌胃1次, 2次/d, 连续灌胃5 d。采用行为学测试(惊厥评估、Longa评分、悬吊实验、水迷宫实验)评估各组大鼠运动神经功能情况和远期学习能力, HE染色与电镜观察脑组织病理结构变化和线粒体损伤情况, 伊文思蓝染色与脑含水量测定用于检测血脑屏障通透性和脑水肿情况, PCR阵列技术检测各组大鼠脑组织中AMPK信号通路相关基因表达情况。结果与模型组比较, 中药组大鼠惊厥发作有所改善、Longa评分降低、握力测试评分与悬吊时间提升、逃避潜伏期缩短、穿越平台次数增加(P<0.05);中药组神经元与线粒体损伤程度减轻, 脑血管通透性与脑含水量降低(P<0.01)。PCR阵列结果显示, 与对照组比较, 模型组脑组织中有2个基因显著上调, 12个基因显著下调;与中药组比较, 模型组有15个基因相对下调。结论活血荣络方可减轻HIBD大鼠脑损伤程度, 改善运动神经功能和提高学习认知能力, 其损伤修复机制与调控AMPK信号通路相关基因表达有关。
Objective To investigate the protective effects of Huoxue Rongluo Decoction on hypoxic-ischemic brain damage(HIBD)rats;To clarify the expression of AMPK signaling pathway during HIBD injury and repair.Methods On the basis of the hypoxia ischemia reperfusion model,an improved hypoxia bottle was used to establish a full-term HIBD rat model.Totally 60 successfully modeled mice were divided into model group and TCM group using a random number table method,with 30 mice in each group and 30 mice in a control group.Two hours before surgery,TCM group was orally administered with Huoxue Rongluo Decoction 1.17 g/100 g(equivalent dose).The control group and model group were orally administered with equal volume of physiological saline.Two hours after surgery,it was orally administered again,twice a day,for consecutive 5 d.Behavioral tests(convulsion assessment,Longa score,suspension test,water maze test)were used to evaluate the motor nerve function and long-term learning ability of young rats in different groups.HE staining and electron microscopy were used to observe the pathological changes of brain tissue and mitochondrial damage.Evans blue(EB)staining and brain water content measurement were used to detect blood-brain barrier permeability and brain edema.The expression of AMPK signaling pathway related genes in brain tissues of different groups of young rats was detected by PCR array technique.Results Compared with the model group,the convulsions of the rats in the TCM group were improved,the Longa score was significantly reduced,the grip strength test score and suspension time were significantly improved,the escape latency was significantly shortened,and the number of crossing platforms significantly increased(P<0.05);the degree of neuronal and mitochondrial damage in the TCM group was reduced,and cerebral vascular permeability and brain water content were significantly reduced(P<0.01).The results of PCR array showed that compared with the control group,2 genes were significantly up-regulated and 12 genes were signifi
作者
沈田丰
甘娜
谢雨青
何楚欣
陈姝静
吴芳莹
Tianfeng Shen;Na Gan;Yuqing Xie;Chuxing He;Shujing Chen;Fangying Wu(Graduate School,Guangxi University of Chinese Medicine,Nanning 530201,China;Department of Pediatrics,Guangxi International Zhuang Medical Hospital,Nanning 530201,China)
出处
《国际中医中药杂志》
2024年第7期881-888,共8页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金(82260952)
广西国际壮医医院引进人才科研启动基金项目(GZ2021RC014)。
关键词
缺氧缺血
脑
活血荣络方
AMPK信号通路
大鼠
Hypoxia-ischemia,brain
Huoxue Rongluo Decoction
AMPK signaling pathway
Rats
