摘要
以金线莲无菌苗的茎段为材料,探究不同植物激素组合及配比对金线莲不定芽的诱导、增殖以及生根的影响,以期获得金线莲最佳的组培快繁体系。结果表明,外源激素的添加对金线莲丛生芽的诱导影响显著,金线莲芽诱导的最佳培养组合为基本培养基(MS)+0.1μmol·L^(-1)萘乙酸(NAA)+20μmol·L^(-1)6-苄基腺嘌呤(6-BA);金线莲生根诱导的最佳培养组合为1/2MS+10μmol·L^(-1)吲哚丁酸(IBA)+2.0 g·L^(-1)活性炭。在草炭、珍珠岩、蛭石体积比为3∶1∶1的混合栽培基质中,组培苗长势最好,成活率90%以上。该研究结果为进一步优化金线莲规模化繁育系数提供技术支持。
In order to obtain the optimal rapid propagation system,we studied the effects of plant hormone combinations and ratios on the induction,proliferation and rooting of adventitious shoots.The results showed that exogenous hormones had a significant effect on the induction of cluster buds.The optimal culture combination for bud induction was MS+0.1μmol·L^(-1) NAA+20μmol·L^(-1)6-BA.The optimal culture combination for rooting induction was 1/2 MS+10μmol·L^(-1) IBA+2.0 g·L^(-1) activated carbon.In the mixed culture medium with a volume ratio of 3∶1∶1 for peat,perlite and vermiculite,the growth of regenerated seedlings was the best,and the survival rate was more than 90%.This study provide technical support for further optimization of the scale breeding of Anoectochilus roxburghii.
作者
包水平
武越
钱婷婷
胡海涛
BAO Shuiping;WU Yue;QIAN Tingting;HU Haitao(Dongyang Wildlife Conservation and Management Station,Jinhua 317600,Zhejiang;College of Life Sciences,Zhejiang Normal University,Jinhua 321004,Zhejiang)
出处
《浙江农业科学》
2024年第7期1588-1591,共4页
Journal of Zhejiang Agricultural Sciences
关键词
金线莲
组织培养
激素
Anoectochilus roxburghii
tissue culture
hormone
