摘要
目的 探讨依达拉奉(EDA)对小鼠单核巨噬细胞白血病细胞RAW264.7炎性反应的抑制作用。方法 以脂多糖诱导RAW264.7细胞,复制炎性细胞模型。采用CCK-8法检测EDA的细胞毒性作用。实验分为空白组(等体积培养基)、模型组(等体积培养基)、给药组(40μg/mL EDA)。采用Griess法检测细胞中一氧化氮(NO)水平,采用酶联免疫吸附(ELISA)法测定细胞中前列腺素E2(PGE2)、白细胞介素1β(IL-1β)、白细胞介素18(IL-18)、肿瘤坏死因子-α(TNF-α)、活性氧(ROS)水平;采用实时荧光定量聚合酶链反应(qPCR)法测定细胞中IL-1β和IL-18 mRNA表达水平;采用Western blot法检测细胞中JAK2/信号转导和转录激活因子3(STAT3)通路相关蛋白JAK2,p-JAK2,STAT3,p-STAT3,IL-1β,IL-18的蛋白表达水平。结果 与0μg/mL比较,20,40,80,160μg/mL EDA处理下细胞存活率无显著变化(P <0.05)。与模型组比较,给药组细胞中NO,PGE2,IL-1β,IL-18,TNF-α,ROS水平均显著降低(P <0.05);IL-1β与IL-18 mRNA及蛋白表达水平和p-JAK2/JAK2与p-STAT3/STAT3均显著降低(P <0.05)。结论 EDA可能通过抑制JAK2/STAT3通路的激活而抑制RAW264.7细胞的炎性反应。
Objective To investigate the inhibitory effect of edaravone(EDA)on the inflammatory reactions of mouse leukemic monocyte macrophage RAW264.7 cells.Methods RAW264.7 cells were induced by lipopolysaccharide to replicate the inflammatory cell model.The CCK-8 method was used to detect the cytotoxic effect of EDA.The RAW264.7 cells were divided into the blank group(equal volume of medium),model group(equal volume of medium),and drug group(40μg/mL EDA).The Griess method was used to detect the nitric oxide(NO)level in cells,the enzyme-linked immunosorbent assay(ELISA)was used to detect the prostaglandin E2(PGE2)and interleukin-1β(IL-1β),interleukin-18(IL-18),tumor necrosis factor-α(TNF-α)and reactive oxygen species(ROS)levels.The real-time fluorescence quantitative polymerase chain reaction(qPCR)was used to detect the expression levels of IL-1βand IL-18 mRNA in cells.The Western blot was used to detect the expression levels of JAK2/signal transducer and activator of transcription 3(STAT3)pathway-related proteins including JAK2,p-JAK2,STAT3,p-STAT3,IL-1βand IL-18.Results Compared with that at 0μg/mL,there was no significant change in survival rate of cells at 20,40,80,160μg/mL EDA(P<0.05).Compared with those in the model group,the NO,PGE2,IL-1β,IL-18,TNF-αand ROS levels in the drug group were significantly lower(P<0.05);the expression levels of IL-1β,IL-18 mRNA and proteins,p-JAK2/JAK2 and p-STAT3/STAT3 in the drug group were significantly lower(P<0.05).Conclusion EDA may inhibit the inflammatory reactions of RAW264.7 cells by inhibiting the activation of the JAK2/STAT3 pathway.
作者
李建芬
刘嘉欣
黄凤蕊
罗球珠
LI Jianfen;LIU Jiaxin;HUANG Fengrui;LUO Qiuzhu(The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine·Guangdong Clinical Research Academy of Chinese Medicine,Guangzhou,Guangdong,China 510405)
出处
《中国药业》
CAS
2024年第14期57-60,共4页
China Pharmaceuticals
基金
广东省中医药局科研项目[20221152]。
