摘要
目的评估自然流产患者蜕膜血管生成情况,并探讨自然流产患者蜕膜中甲状腺激素受体信号的表达改变。方法收集行人流术的孕妇蜕膜。以自然流产患者作为实验组,以社会因素行人流术的健康孕妇作为对照组,收集两组人群蜕膜组织。采用Western blot方法,检测蜕膜组织中甲状腺激素受体-α(THR-α)和THR-β的核转位情况,同时使用免疫组化法定位蜕膜组织中THR-α和THR-β的分布。Western blot测定蜕膜组织中Ⅱ型脱碘酶(DIO2)的蛋白表达水平,免疫荧光法定位DIO2在蜕膜组织分布并测定荧光强度。使用免疫组化,以CD34作为内皮标志物测定蜕膜血管密度。Western blot检测血管内皮生长因子受体(VEGFR-1)的蛋白表达水平。结果对照组与流产组在年龄、妊娠天数、TSH、T3、T4等基本资料中,差异均无统计学意义。Western blot结果提示THR-α和THR-β蛋白核转位置下降,同时蜕膜组织免疫组化染色结果表明自然流产患者蜕膜甲状腺激素受体核转位减少(P<0.05)。Western blot结果表明流产组DIO2蛋白表达较对照组下降,提示自然流产孕妇蜕膜中,甲状腺激素代谢受到影响;免疫荧光实验结果显示流产组DIO2的荧光强度较对照组减弱(P<0.05)。免疫组化法CD34标记蜕膜小血管的结果显示,在流产组中,CD34^(+)血管数量下降,差异有统计学差异(P<0.05)。Western blot结果提示流产组和对照组中VEGFR-1无明显变化,差异无统计学意义。结论蜕膜局部甲状腺激素受体信号异常可能导致蜕膜血管生成减少,从而参与自然流产的发生。
Objective To evaluate the angiogenesis of decidua in spontaneous abortion patients and to investigate the change of thyroid hormone receptor signal expression in decidua in miscarriage patients.Methods The decidua of pregnant women was collected.Patients with miscarriage were taken as the experimental group and healthy pregnant women who underwent abortion by social factors were taken as the control group.The nuclear translocation of thyroid hormone receptorα(THR-α)and THR-βin decidua tissues was detected by Western blot method,and the distribution of THR-αand THR-βin decidua tissues was determined by immunohistochemistry.The expression of Deiodinase 2(DIO2)in decidua tissues was determined by Western blot.The distribution of DIO2 in decidua tissues was determined by immunofluorescence and the fluorescence intensity was measured.The decidual vessel density was determined by immunohistochemistry using CD34 as an endothelial marker.The protein expression of vascular endothelial growth factor receptor(VEGFR-1)was detected by Western blot.Results There was no significant difference in age,gestation days,TSH,T3,T4 and other basic data between the control group and the abortion group.The results of Western blot showed that the expression levels of THR-αand THR-βprotein decreased,and the results of immunohistochemical staining of decidual tissues showed that the nuclear translocation of decidual thyroid hormone receptor in patients with spontaneous abortion decreased(P<0.05).Western blotting results showed that the expression of DIO2 protein in the abortion group was lower than that in the control group,suggesting that thyroid hormone metabolism in decidua of spontaneous abortion pregnant women was affected.The results of immunofluorescence test showed that the fluorescence intensity of DIO2 in the abortion group was weaker than that in the control group(P<0.05).Immunohistochemical CD34 labeling of decidual small blood vessels showed that the number of CD34^(+)blood vessels decreased in the abortion group with st
作者
翟沐鑫
李萱
余涛
赵丽
于震
杨媛媛
Zhai Muxin;Li Xuan;Yu Tao;Zhao Li;Yu Zhen;Yang Yuanyuan(Dept of Obstetrics and Gynecology,The First Affiliated Hospital of Anhui Medical University,Hefei 230032;NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract(Anhui Medical University),Hefei 230032;Anhui Province Key Laboratory of Reproductive Health and Genetics,Hefei 230032)
出处
《安徽医科大学学报》
CAS
北大核心
2024年第6期1076-1081,共6页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金项目(编号:2008085MH243)。
关键词
流产
蜕膜化
甲状腺激素受体
miscarriage
decidualization
thyroid hormone receptor
