摘要
试验旨在探讨犬葡萄糖激酶调节蛋白(glucokinase regulatory protein,GCKR)的结构、功能及表达。试验采用生物信息学方法对犬GCKR蛋白的理化性质、亲/疏水性、同源性和保守基序、信号肽和跨膜区、二级结构、结构域、三级结构、磷酸化修饰、相互作用蛋白质和GO注释、亚细胞定位、基因结构、启动子和转录调控进行了研究。结果显示:犬GCKR蛋白是一种不稳定酸性亲水蛋白质,与马的亲缘关系较近,没有信号肽和跨膜区;二级结构由α-螺旋(47.36%)、延伸链(12.00%)、β-转角(4.48%)和无规则卷曲(36.16%)构成,含有2个糖异构酶结构域;三级结构以马GCKR蛋白为模板构建且质量较高。犬GCKR蛋白含有28个磷酸化位点,其相互作用蛋白包括葡萄糖激酶、酰胺水解酶结构域2、锌指蛋白512等,并参与糖代谢,主要定位于内质网;其基因具有19个外显子和18个内含子,启动子位于第1 302~1 352 bp,转录因子如Ncx、POU1F1a、C/EBPdelta可与启动子结合。研究表明,犬GCKR蛋白可能参与犬糖尿病的发生机制,可作为研发相关药物的潜在靶标。
The purpose of the study was to investigate the structure,function and expression of canine GCKR protein.Bioinformatics methods were used to study the physicochemical properties,hydrophilicity/hydrophobicity,homology and conserved motif,signal peptide and transmembrane region,secondary structure,domain,tertiary structure,phosphorylation modification,interacting protein and GO annotation,subcellular localization,gene structure,promoter and transcriptional regulation of canine GCKR protein.The results showed that canine GCKR protein was an unstable acidic hydrophilic protein,which was closely related to horses and had no signal peptide and transmembrane region.The secondary structure consists ofα-helix(47.36%),extended chain(12.00%),β-turn angle(4.48%),and random coil(36.16%),and it contains two sugar isomerase domains.The tertiary structure is constructed with horse GCKR protein as template and has high quality.There are 28 phosphorylation sites,and the interacting proteins include glucokinase,amide hydrolase domain 2,zinc finger protein 512,etc.It is involved in glucose metabolism and is mainly located in the endoplasmic reticulum.Its gene has 19 exons and 18 introns,and the promoter is located at 1302~1352 bp.Transcription factors such as Ncx,POU1F1a,C/EBPdelta can bind to the promoter.Studies have shown that canine GCKR protein may be involved in the pathogenesis of canine diabetes,and can be used as a potential target for developing related drugs.
作者
刘迪瑄
袁鸣欣
肖鹏硕
刘蕊
刘博远
庞若玉
宋书峰
马学婧
Liu Dixuan;Yuan Mingxin;Xiao Pengshuo;Liu Rui;Liu Boyuan;Pang Ruoyu;Song Shufeng;Ma Xuejing(Department of Life Sciences,Cangzhou Normal University,Hebei Cangzhou 061001)
出处
《现代畜牧兽医》
2024年第4期17-24,共8页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
沧州师范学院大学生科技创新能力培育专项(DXSYBL2023007)
沧州师范学院校内科研基金项目(XNJJLQN2021002)
沧州师范学院科研创新团队(cxtdl2303)
河北省教育厅科学技术研究项目(QN2020515)
沧州市自然科学基金项目(221001002D)。
