摘要
目的:为肥胖症和2型糖尿病的手术和内镜介入治疗研究建立一种大型动物实验模型.方法:选用10只健康♂成年Beagle犬,随机分成两组,对照组5只全程(16wk)给予标准饲料喂养,模型组5只全程给予高脂高热量饲料喂养,并于第8周给予小剂量链脲佐菌素(STZ)静脉注射.定时检测动物体质量、体长、空腹血糖、静脉葡萄糖实验(IVGTT)、血脂等与肥胖症及2型糖尿病相关临床指标.结果:在第8周,模型组平均体质量是16.9kg±0.8kg,较对照组体质量(14.8kg±1.03kg)有显著升高(P<0.01),Lee指数与对照组比较也有显著差异(P<0.05);模型组血清胰岛素、C-肽较对照组明显上升(P<0.01).STZ注射后1wk,模型组空腹血糖明显高于对照组(P<0.05),IVGTT-2h血糖比注射前增高(143mg/dL±7.4mg/dLvs127mg/dL±4.2mg/dL,P<0.05),血清胰岛素、C-肽分泌比注射前明显下降(P<0.01).STZ注射后8wk,模型组空腹血糖显著高于对照组(P<0.01),IVGTT-2h血糖较注射前显著增高(323mg/dL±12.1mg/dLvs127mg/dL±4.2mg/dL,P<0.01).本实验模型组Beagle犬成模率为100%,实验期间Beagle犬未见死亡.结论:联合高脂高热量喂养和小剂量STZ注射可在Beagle犬上成功建立肥胖症并2型糖尿病动物模型,此模型接近人类2型糖尿病的发病过程和代谢特征,可用于肥胖症和2型糖尿病的手术和内镜介入治疗的研究.
AIM：To establish a big animal model of obesity and type 2 diabetes for further investigation of surgical or endoscopic interventional therapy of this disease.METHODS：Ten healthy adult male Beagle dogs were randomly and equally divided into two groups：control group and model group.The control group was feed a standard diet during the whole experiment（16 wk）,while the model group was feed a high-fat hypercaloric diet.At week 8,the model group was intravenously injected with small dose of streptozotocin（STZ）.The body mass,length,fasting blood glucose,intravenous glucose tolerance test（IVGTT）,andlipid were measured regularly.RESULTS：At week 8,the average body mass（16.9 kg ± 0.8 kg vs 14.8 kg ± 1.03 kg,P 0.01）,Lee index（P 0.05）,serum insulin and c-peptide（both P 0.01） were significantly higher in the model group than in the control group.One week after STZ injection,in the model group,the fast blood glucose was signif icantly higher than that in the control group（P 0.05）,IVGTT-2 h glucose was significantly higher than the preinjection value（143 mg/dL ± 7.4 mg/dL vs 127 mg/dL ± 4.2 mg/dL,P 0.05）,and serum insulin and c-peptide decreased significantly（P 0.01）.Eight weeks after STZ injection,in the model group,the fast blood glucose was signif icantly higher than that in the control group（P 0.01）,and IVGTT-2h glucose was signif icantly higher than the pre-injection value（323 mg/dL ± 12.1 mg/dL vs 127 mg/dL ± 4.2 mg/dL,P 0.01）.All Beagle dogs were confirmed to have type 2 diabetes,and no Beagle dogs died during the experimental period.CONCLUSION：A high-fat hypercaloric diet combined with intravenous injection of small dose of STZ can induce obesity and type 2 diabetes in Beagle dogs.This animal model mimics the nature history and metabolic characteristics of human type 2 diabetes and can be used for further investigation of surgical or endoscopic interventional therapy of obesity and type 2 diabetes.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第24期2534-2539,共6页
World Chinese Journal of Digestology
