摘要
为了解抹茶菌群多样性,以不同企业不同等级的抹茶为试验材料,采用CTAB法提取样品总DNA,分别测定样品中微生物的16SrDNA基因V3、V4区序列。测序数据经质量控制、嵌合体过滤、高质量数据统计后,利用QIIME2软件获得扩增子序列变体ASVs特征表和特征序列,进行ASVs分布、α多样性、β多样性、物种和物种关系分析。结果表明,45个抹茶样本中有9个样本的菌群丰度极低,集中在高等级产品中。Z企业和Y企业的抹茶菌群ASVs数目、Chao1和Observed-species指数均与抹茶等级呈负相关性,而J企业抹茶的上述指标与抹茶等级不成相关性。Shannon指数表明影响各抹茶中菌群多样性的因素基本一致。抹茶组内样本间群落结构相近,企业内部样本间群落结构相近,同一企业不同批次但等级相同的样本菌群结构相近,产品微生态稳定性较好。36个抹茶样中共检测到物种29门、71纲、176目、345科、902属、1577种,其中变形菌门(Proteobacteria)和厚壁菌门(Firmicutes)为各组样本的绝对优势菌门,二者在ZA、ZB、ZC、YA、YB、JA、JB和JC中的丰度之和分别为96.34%、95.85%、96.06%、95.53%、95.31%、95.91%、96.06%和95.27%。鞘氨醇单胞菌属(Sphingomonas)、甲基杆菌属(Methylobacterium)和假单胞杆菌属(Pseudomonas)为各组的共有优势菌属(丰度≥2.00%),优势菌属在上述各组中的丰度之和分别为64.71%、65.61%、60.04%、70.65%、69.20%、72.98%、75.92%和76.57%。各组的共有优势菌属均属于变形菌门(Proteobacteria),ZB的第一优势菌属链球菌属(Streptococcus)属于厚壁菌门(Firmicutes)。本试验结果为阐明抹茶菌群多样性提供理论参考。
In order to understand the microbial diversity of matcha,different levels of matcha from different enterprises were used as experimental materials,the total DNA of the samples was extracted using the CTAB method,and the V3 and V4 regions sequences of the 16SrDNA gene of the microorganisms in the samples were determined,respectively.After quality control,chimera filtering and high quality data statistics on sequencing data,the amplified sub sequence variant ASVs feature table and feature sequences were obtained by QIIME2 software,and the ASVs distribution,αandβdiversity,species and species relationships analysis were performed.The results showed that 9 out of 45 matcha samples had extremely low microbial abundance,concentrated in high-grade macha products.The number of ASVs,Chao1 and Observed-species index in the microbial community of matcha from Z and Y enterprises were negatively correlated with matcha grade;However,the above indexes of J company's matcha had no correlation with the matcha grade;The shannon index showed that the factors affecting the microbial diversity of each matcha were basically the same.The community structure of the samples within the matcha group was similar,the community structure of the samples within the enterprise samples was similar,the microbial community structure of the samples of different batches but the same grade from the same enterprise was similar,and the product’s microecological stability was good.A total of 29 phylums,71 classes,176 orders,345 families,902 genera and 1577 species were detected in 36 matcha samples.Proteobacteria and Firmicutes were the absolute dominant phyla in each group of samples.The sum of the dominant phyla abundances in ZA,ZB,ZC,YA,YB,JA,JB and JC were 96.34%,95.85%,96.06%,95.53%,95.31%,95.91%,96.06%and 95.27%,respectively.Sphingomonas,Methylobacterium and Pseudomonas were the common dominant genera in each group(abundance≥2.00%).The sum of abundance of the dominant genera in the above groups was 64.71%,65.61%,60.04%,70.65%,69.2%,72.98%,75.9
作者
蒋玉兰
朱跃进
吕杨俊
潘俊娴
叶丽伟
王霈菲
张士康
JIANG Yulan;ZHU Yuejin;LYU Yangjun;PAN Junxian;YE Liwei;WANG Peifei;ZHANG Shikang(Hangzhou Tea Research Institute,All China Federation of Supply and Marketing Cooperatives/Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resources,Hangzhou 310016,China)
出处
《茶叶通讯》
2024年第2期210-219,242,共11页
Journal of Tea Communication
基金
浙江省“尖兵”“领雁”研发攻关计划项目(2022C02060)。
