摘要
为研究热致自卷曲与生长因子梯度化修饰对血管内皮化的促进作用,使用左旋聚乳酸(PLLA)和聚乳酸-羟基乙酸共聚物(PLGA)作为材料,通过静电纺丝技术制备了具有热致自卷曲特性的PLLA/PLGA纳米纤维血管支架。通过静电喷雾技术制备了梯度生长因子修饰血管支架内层,并对其自卷曲、微观结构、生物相容性及内皮化功能进行表征。结果表明:制备的PLLA/PLGA血管支架具备多层取向纳米纤维结构,厚度为(6.75±0.4)μm,具有出色的生物相容性,可在37℃条件下自卷曲成管状结构;血管支架内层膜对生长因子成功进行了梯度修饰,修饰后血管支架的细胞迁移距离是未修饰的3.5倍,从而加快了内皮细胞迁移,促进血管内层的快速内皮化。
Objective At present,self-coiling technology is rarely used in the field of blood vessels.Thermally induced self-coiling can make vascular tissue engineering materials self-coil at the corresponding temperature to wrap the injured blood vessels,which has a better fit compared with the existing artificial blood vessels.At the same time,self-coiling is irreversible,and the hardness of tissue engineering materials is enhanced after self-coiling.In orderto solve the problem of vascular endothelialization,gradient modification of growth factors was applied to the surface of the thermally induced self-coil ing scaffolds to promote rapid endothelialization of the inner layer of the scaffold.At the same time,the scaffold has a multi-layer microstructure,which simulates the fiber direction of each layer of the blood vessel and has bionic performance.Method Poly(l-lactic acid)(PLLA)and poly(lactic-co-glycolic acid)(PLGA)were used as raw materials to fabricate thermally induced self-coiling PLLA/PLGA nanofibrous vascular scaffolds by electrospinning technology.The thickness of the scaffolds was measured by a micrometer.The scaffold can self-coil at 37℃,and the driving force of self-coil comes from the difference in molecular motion rate between PLLA and PLGA after heating.The surface morphology of PLLA and PLGA nanofibrous membranes was observed by SEM.The gradient growth factor modification of the inner layer of the scaffold was made by electrostatic spraying technology and different mold coverings,and the gradient preparation was observed under fluorescence microscope by replacing vasular endothelial growth factor(VEGF)with rhodamine.The cytocompatibility of scaffolds was tested by CCK-8 assay.The effect of gradient-modified growth factors on rapid endothelialization was examined by seeding cells on both sides of the scaffold and observing them by fluorescent staining after 3 d.Results The prepared PLLA/PLGA vascular scaffold had a multi-layer oriented nanofiber structure.The microstructures of the inner PLGA and the ou
作者
于承浩
王元非
于腾波
吴桐
YU Chenghao;WANG Yuanfei;YU Tengbo;WU Tong(Medical College,Qingdao University,Qingdao,Shandong 266071,China;Qingdao Municipal Hospital,Qingdao,Shandong 266071,China;Institute of Neuroregeneration and Neurorehabilitation,Qingdao University,Qingdao,Shandong 266071,China)
出处
《纺织学报》
EI
CAS
CSCD
北大核心
2024年第7期18-23,共6页
Journal of Textile Research
基金
山东省自然科学基金青年项目(ZR2021QC063)。
