摘要
【目的】探究树木防冻防病膜剂的抑菌效果,为生产提供一种良好的防冻保护剂。【方法】利用课题组研制的树木防冻防病膜剂对2种冰核细菌(丁香假单胞菌和菠萝泛生氏菌)和3种真菌(小穴壳菌属、金黄壳囊孢菌、污黑腐皮壳菌)进行抑菌效果研究,并与市面常用药剂进行比较。采用滤纸片扩散法对比3个浓度(0.3‰、0.6‰和1‰)下农用链霉素和防冻防病膜剂对两种冰核细菌抑制效果。采用生长速率法对比4个浓度(100 mg·L^(-1)、300 mg·L^(-1)、600 mg·L^(-1)和1000 mg·L^(-1))下多菌灵、甲基硫菌灵、百菌清和防冻防病膜剂4种药剂对3种引起杨树溃疡病和烂皮病真菌的抑制效果。【结果】(1)与农用链霉素相比,防冻防病膜剂对2种冰核细菌的抑制效果较好,且抑菌效果随着药剂浓度的增大而增大。在设置的最高试验浓度下,防冻防病膜剂对丁香假单胞菌和菠萝泛生氏菌的抑菌直径分别为10.9 mm和19.1 mm。(2)多菌灵和甲基硫菌灵对3种真菌抑制效果较好,多菌灵在100 mg·L^(-1)浓度下对3种真菌抑制率能达到100%,甲基硫菌灵在100 mg·L^(-1)浓度下对2种真菌(金黄壳囊孢菌、污黑腐皮壳菌)抑制率能达到100%,在600 mg·L^(-1)浓度下对小穴壳菌属抑制率能达到100%。(3)防冻防病膜剂与百菌清对3种真菌的抑制效果相当,防冻防病膜剂的抑制效果随着药剂浓度增大而增强,当浓度达到1000 mg·L^(-1)时,抑菌率为100%。【结论】树木防冻防病膜剂对2种冰核细菌和3种真菌均有较好抑制效果。
【Objective】To explore the bacteriostatic effect of anti-freezing and disease-preventing film agent for trees,and to provide a good anti-freezing protective agent for production.【Method】The antibacterial effects of two ice nucleated bacteria(Pseudomonas syringae and Pantoea ananatis)and three fungi(Dothiorella grearia,Cytospora chrysosperma,Valsa sordida)were studied by using the antifreeze and disease prevention film agent developed by our research group,and the results were compared with those commonly used in the market.The effect of agricultural streptomycin and antifreeze and disease prevention film on the two ice nucleation bacteria at three concentrations(0.3‰,0.6‰and 1‰)was compared by filter paper diffusion method.Growth rate method was used to compare the inhibitory effects of carbendazim,thiophanate-methyl,chlorothalonil and antifreeze and antidisease film at 4 concentrations(100 mg·L^(-1),300 mg·L^(-1),600 mg·L^(-1) and 1000 mg·L^(-1))on 3 species of poplar canker and rot causing fungi.【Result】(1)Compared with agricultural streptomycin,the antifreezing and disease-preventing film agent had better inhibitory effect on the two ice nucleated bacteria,and the inhibitory effect increased with the increase of the concentration of the agent.Under the maximum experimental concentration,the antibacterial diameter of antifreeze and antidisease film against Pseudomonas syringae and Pantoea ananatis were 10.9 mm and 19.1 mm,respectively.(2)Carbendazim and thiophanate-methyl had a good inhibitory effect on three fungi,with the inhibitory rate of carbendazim reaching 100%at a concentration of 100 mg·L^(-1),thiophanate-methyl reaching 100%at a concentration of 100 mg·L^(-1) against two fungi(Cytospora chrysosperma and Valsa sordida),and at a concentration of 600 mg·L^(-1),the inhibition rate against Dothiorella grearia can reach 100%.(3)The inhibition effect of the anti-freezing and disease-preventing film was equivalent to that of chlorothalonil on three fungi.The inhibition effect of the an
作者
李晓宇
张蕾
杨成超
LI Xiaoyu;ZHANG Lei;YANG Chengchao(Liaoning Institute of Poplar Research,Gaizhou 115200,Liaoning;Shenyang Rural Revitalization and Development Center,Shenyang 110034,Liaoning)
出处
《温带林业研究》
2024年第2期37-44,共8页
Journal of Temperate Forestry Research
基金
辽宁省应用基础研究计划(2023JH2/101300162)。
关键词
防冻防病膜
抑菌效果
冰核细菌
真菌
anti-freezing and disease-preventing film
bacteriostatic effect
ice nucleation active bacteria
fungi
