摘要
目的:研究三桠苦提取物的抑菌活性。方法:以95%乙醇为溶剂,分别利用超声波提取和回流提取方法获得三桠苦提取物,利用滤纸片扩散法,分析两种提取方法的提取物对大肠杆菌、金黄色葡萄球菌、枯草杆菌和沙门氏菌的抑菌活性差异,确定抑菌活性较好的提取方法。利用该方法提取三桠苦得到提取物,采用溶剂萃取法,依次用石油醚、二氯甲烷、乙酸乙酯和正丁醇分别萃取三桠苦提取物,得到极性大小不同的萃取物,再次利用滤纸片扩散法,比较这些萃取物对四种菌的抑菌活性,确定三桠苦的抑菌活性部位,为三桠苦抑菌活性成分的深入研究提供参考。结果:回流提取法获得的样品抑菌活性优于超声提取法;石油醚、二氯甲烷萃取物表现出较好的抑菌活性,乙酸乙酯萃取物抑菌活性较差、正丁醇萃取物无抑菌活性。结论:三桠苦提取物具有抑菌活性,其二氯甲烷萃取物是具有较强抑菌作用的活性部位。
Objective:To evaluate the anti-bacterial activity of the extract of Melicope pteleifolia.Method:The 95%ethanol was used as the solvent,and the extracts of Melicope pteleifolia were prepared by ultrasonic extraction and reflux extraction,respectively.The difference of antibacterial activity of two extracts against Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Salmonella was analyzed by filter paper diffusion method,and the extraction method with better antibacterial activity was selected.The extracts were further fractionated with petroleum ether,dichloromethane,ethyl acetate and n-butanol,and the extracts with different polarity are obtained.Then the filter paper diffusion method was used to compare the antibacterial activities of these extracts against four kinds of bacteria,and determine the antibacterial active components of the medicinal plant,providing a reference for further research on the antibacterial active components of the tree.Result:The antibacterial activity of the samples obtained by reflux extraction was better than that by ultrasonic extraction;The petroleum ether and dichloromethane extracts showed good antibacterial activity,while the ethyl acetate part had poor antibacterial activity,and the n-butanol part had no antibacterial activity.Conclusion:The extract of Sanyaku possess antibacterial activity,and its dichloromethane extract is the active component with strong antibacterial effect.
作者
褚晨亮
欧阳东菊
陈润柯
陈秋怡
梁华炎
黎清生
金路
Chu Chenliang;Ouyang Dongju;Chen Runke;Chen Qiuyi;Liang Huayan;Li Qingsheng;Jin Lu(Department of Pharmaceutical Engineering,Zhaoqing University,Zhaoqing526061,China;School of Pharmacy,Sun Yat-sen University,Guangzhou 510006,China)
出处
《山东化工》
CAS
2024年第4期30-32,36,共4页
Shandong Chemical Industry
基金
广东省青年基金项目,项目编号:2022A1515110734
大学生创新项目,项目编号:X202210580147
肇庆市科技创新项目,项目编号:2022040311011。
关键词
三桠苦
提取物
抑菌
活性部位
Melicope pteleifolia
extractive
bacteriostasis
bioactive component
