摘要
测定了从紫萼玉簪花中萃取分离获得的5种提取物对小菜蛾的杀虫活性,并分析了活性较好的提取物的化学成分。结果表明:正丁醇萃取物对小菜蛾的生物活性较好,氯仿萃取物生物活性次之,石油醚萃取物、乙酸乙酯萃取物和水提取物的生物活性较弱。其中质量浓度为250 mg/L正丁醇萃取物对小菜蛾处理12 h后,拒食活性达到98%;处理24 h后触杀活性为83.33%;处理48 h后熏蒸活性和胃毒活性分别为53.63%和57.78%;在同一浓度下驱避活性达到90.48%。通过GC-MS分析从正丁醇萃取物中鉴定出28种化学成分,主要成分为棕榈酸(17.189%)、亚油酸(10.041%)、丁酸丁酯(8.641%)、亚麻酸(8.53%)、棕榈酸丁酯(4.56%)等。
In this study,five kinds of extracts obtained from the flowers of Hosta ventricosa were measured for insecticidal activity against Plutella xylostella,and the chemical components of the extracts with insecticidal activity were analyzed.The results showed that the n-butanol extract had the best biological activity against P.xylostella.The biological activity of chloroform extract was second,and the biological activities of petroleum ether extract,ethyl acetate extract and water extract were weak.The antifeedant activity reached 98% at a concentration of 250 mg/L after 12 hours of treatment,the contact activity was 83.33% after 24 hours of treatment,the fumigation activity and stomach poisoning were 53.63% and 57.78% after 48 hours of treatment.The repellent activity reached 90.48% at the same concentration.Twenty-eight chemical components were identified from the n-butanol extract through GC-MS analysis,and the main components were palmitic acid(17.189%),linoleic acid(10.041%),linolenic acid(8.53%),butyl butyrate(8.641%),and butyl palmitate(4.56%).
作者
王艺卉
毕增一
陈思妤
韩东昊
黄钰麟
袁海滨
WANG Yihui;BI Zengyi;CHEN Siyu;HAN Donghao;HUANG Yulin;YUAN Haibin(College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;International Department of the High School Attached to Northeast Normal University,Changchun 130024,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2024年第1期28-33,共6页
Journal of Jilin Agricultural University
基金
国家自然科学基金项目(31101440)
吉林农业大学国家级大学生创新创业训练计划项目(201810193035)。
关键词
紫萼玉簪
小菜蛾
杀虫活性
成分分析
萃取分离
Hosta.ventricosa
Plutella xylostella
insecticidal activity
component analysis
extraction separation
