摘要
研究利用超声辅助提取黑枸杞多糖,以其得率为考察指标,对料液比、提取温度、提取时间和超声功率4个因素进行单因素试验,再进一步进行四因素三水平的正交试验优化黑枸杞多糖的提取工艺参数,并对超声辅助提取得到的黑枸杞多糖(LRP-U)与热水浸提法所得黑枸杞多糖(LRP-H)进行抗氧化和抗肿瘤活性对比分析。结果表明,超声辅助提取黑枸杞多糖的最佳提取条件为料液比1∶30 g/mL、提取温度70℃、提取时间70 min和超声功率300 W,在此条件下提取率为24.53%。所得多糖对DPPH·、OH·和ABTS+·的清除率最大分别达到82.52%,35.81%和76.68%,均高于LRP-H。并且对抑制肝癌细胞HepG2具有显著效果, LRP-U和LRP-H的最高抑制率分别达到34.31%和31.04%。以上结果显示,超声辅助提取的黑枸杞多糖具有良好的体外抗氧化和抗肿瘤活性。
In this study, ultrasonic-assisted extraction of Lycium ruthenicum polysaccharide was used, and its yield was taken as the index. Single factor tests were conducted on solid-liquid ratio, extraction temperature, extraction time and ultrasonic power, and four-factor and three-level orthogonal tests were further carried out to optimize the extraction process parameters of Lycium ruthenicum polysaccharide. The antioxidant and antitumor activities of Lycium ruthenicum polysaccharide extracted by ultrasound(LRP-U) and extracted by hot water extraction(LRP-H) were compared. The results showed that the optimal extraction conditions were as follows: solid-liquid ratio 1∶30 g/mL, extraction temperature 70 ℃, extraction time 70 min and ultrasonic power 300 W, under which the extraction yield was 24.53%. The maximum clearance rates of DPPH·, OH· and ABTS+· were 82.52%, 35.81% and 76.68%, respectively, which were higher than those of LRP-H. Moreover, the inhibition rate of LRP-U and LRP-H reached 34.31% and 31.04%, respectively. The above results showed that the polysaccharides extracted by ultrasonic-assisted extraction had good anti-oxidation and anti-tumor activities in vitro.
作者
佟欣
TONG Xin(The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000)
出处
《食品工业》
CAS
2024年第5期135-140,共6页
The Food Industry
基金
KASSF10在左右半结肠癌组织中的差异性表达及临床意义研究(19B1D34)。
关键词
超声
黑枸杞多糖
抗氧化
抗肿瘤
ultrasound
Lycium ruthenicum polysaccharide
antioxidant
antitumor
