摘要
目的初步评价低压低氧对创伤后应激障碍(post-traumatic stress disorder,PTSD)大鼠机体损伤的影响。方法将大鼠随机分为4组,空白对照(Control)组、单程长时应激(single-prolonged stress,SPS)组、低压低氧(hypobaric hypoxia,HH)组和单程长时应激复合低压低氧(SPS+HH)组。首先运用SPS方法对SPS组和SPS+HH组大鼠进行PTSD模型构建,第2天将HH组和SPS+HH组大鼠放入低压低氧舱,模拟海拔6000 m,持续14 d。通过检测大鼠一般状况、行为学、血液学指标和组织形态学,以评价低压低氧对PTSD大鼠的损伤。结果SPS+HH组大鼠体质量显著下降;粪便部分偏硬结,部分可见粘度大。除Control组外,其余各组均出现焦虑样和抑郁样行为,其中低压低氧加重SPS大鼠的行为学异常。SPS组和SPS+HH组大鼠凝血功能障碍,血液粘稠度异常增加,SPS+HH组显著异常;SPS+HH组大鼠全血中红细胞、血红蛋白和红细胞比容均较SPS组大鼠显著增加;SPS+HH组大鼠血清TP、LDH和GLU含量异常。组织形态学方面,SPS+HH组大鼠海马组织可见扩张充血的血管,肝脏组织可见充血的中央静脉,肝窦可见扩张充血、肝细胞轻度颗粒样变。结论低压低氧加重PTSD大鼠的抑郁样和焦虑样行为,同时加重PTSD大鼠的血液学指标和组织形态学异常。
Aim To preliminarily evaluate the effects of hypobaric hypoxia on organism damage in rats with post-traumatic stress disorder(PTSD),with a view to laying a foundation for drug research in plateau PTSD.Methods The rats were randomly divided into four groups,namely,the control(Control)group,the single-prolonged stress(SPS)group,the hypobaric hypoxia(HH)group and the single-prolonged stress combined with hypobaric hypoxia(SPS+HH)group.The PTSD model was firstly constructed using the SPS method for rats in the SPS and SPS+HH groups.On the second day,rats in the HH group and SPS+HH group were placed in a low-pressure hypoxia chamber at a simulated altitude of 6000 m for 14 days.General condition,behavior,blood tests,and histomorphology were examined in order to evaluate the damage caused by low pressure hypoxia in PTSD rats.Results The body mass of rats in the SPS+HH group was significantly reduced;the feces were partly hard and lumpy,and some of them were seen to have high viscosity.Anxiety-like and depression-like behaviors were observed in all groups except in the control group,in which hypobaric hypoxia aggravated the behavioral abnormalities in SPS rats.Rats in both the SPS and SPS+HH groups had coagulation dysfunction and abnormally increased blood viscosity,which was significantly abnormal in the SPS+HH group;erythrocytes,hemoglobin,and erythrocyte specific volume in whole blood of rats in the SPS+HH group were significantly increased compared with those of rats in the SPS group;and serum TP,LDH and GLU levels were abnormal in rats in the SPS+HH group.Dilated and congested blood vessels were seen in hippocampal tissue,congested central veins were seen in hepatic tissue,and dilated and congested liver sinusoids with mild granulomatous degeneration of hepatocytes were seen in rats of the SPS+HH group.Conclusion Hypobaric hypoxia exacerbates depression-like and anxiety-like behaviors in PTSD rats,as well as hematological indices and histomorphometric abnormalities in PTSD rats.
作者
沈宝英
王智星
李博尉
杨春启
沈歆
赖成材
高月
SHEN Bao-ying;WANG Zhi-xing;LI Bo-wei;YANG Chun-qi;SHEN Xin;LAI Cheng-cai;GAO Yue(Guangdong Pharmaceutical University,Guangzhou 510006,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China;Qinghai University,Xining 810016,China;Chinese PLA Medical School,Beijing 100853,China;Faculty of Environment and Life Science,Beijing University of Technology,Beijing 100022,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2024年第7期1231-1239,共9页
Chinese Pharmacological Bulletin
基金
国家中医药管理局中医药创新团队及人才支持计划项目(No ZYYCXTD-D-202207)。
关键词
低压低氧
创伤后应激障碍
行为学
血液学指标
海马组织
肝脏组织
hyperbaric and hypoxic
post-traumatic stress disorder
behavioral
hematological indicators
hippocampus
liver tissue
