摘要
目的探讨黄连素对糖尿病(diabetes mellitus,DM)大鼠血脂代谢及胰岛素抵抗(insulin resistance,IR)的作用。方法取10只正常大鼠作为正常对照组(Con)。将DM建模成功大鼠随机分为模型组(M)、阳性对照组(Pon)、黄连素低剂量组(L-Ber)和黄连素高剂量组(H-Ber),分别为9、9、9、8只,将剩余8只非DM模型大鼠纳入非DM组(N-DM)。经相应干预后,测定各组大鼠体质量、饮水量、摄食量、血糖、血脂指标和IR;HE染色观察胰腺组织形态学变化;蛋白印迹法(Western blotting)检测磷酸化胰岛素受体底物1(phosphorylated insulin receptor substrate 1,p-IRS-1)、磷酸化磷脂酰肌醇3激酶(phosphorylated phosphatidylinositol 3-kinase,p-PI3K)和葡萄糖转运蛋白-4(glucose transporter-4,GLUT-4)蛋白的表达水平。结果与Con组比较,M组的饮水量、摄食量、总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、空腹胰岛素(fasting insulin,FIns)、胰岛素抵抗指数(homeostasis model assessment of insulin resistance,HOMA-IR)、糖耐量和空腹血糖(fasting blood glucose,FBG)均升高,体质量、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、p-IRS-1、p-PI3K和GLUT-4蛋白相对表达量均降低(P<0.05)。与M组比较,L-Ber组、H-Ber组和Pon组的饮水量、摄食量、TC、TG、LDL-C、FIns、HOMA-IR、糖耐量和FBG均降低,胰腺组织形态学异常减轻,体质量、HDL-C、p-IRS-1、p-PI3K和GLUT-4蛋白相对表达量均升高(P<0.05)。结论黄连素可改善DM大鼠血脂代谢及IR,作用机制可能与调节IRS-1/PI3K/GLUT4通路有关。
Objective To investigate the effect of berberine on blood lipid metabolism and insulin resistance(IR)in diabetes mellitus(DM)rats.Methods Ten normal rats were taken as the normal control group(Con).The rats with successful DM modeling were randomly divided into DM model group(M),positive control group(Pon),berberine low(L-Ber)and high-dose groups(H-Ber),which were 9,9,9 and 8 respectively.The other 8 non-DM rats were included in the non-DM group(N-DM).After the corresponding intervention,the body weight,drinking water,food intake,blood glucose,blood lipid and IR were measured.The histomorphological changes of pancreas were observed by HE staining.The protein expressions of phosphorylated insulin receptor substrate 1(p-IRS-1),the protein expression of phosphorylated phosphatidylinositol 3-kinase(p-PI3K)and glucose transporter-4(GLUT-4)were detected by Western blotting.Results Compared with the Con group,the water intake,food intake,total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),fasting insulin,(FIns),homeostasis model assessment of insulin resistance,(HOMA-IR),glucose tolerance,fasting blood glucose(FBG)increased,the body weight,high density lipoprotein cholesterol(HDL-C)and the relative expression of p-IRS-1,p-PI3K and GLUT-4 protein decreased(P<0.05).Compared with the M group,the water intake,food intake,TC,TG,LDL-C,FIns,HOMA-IR,glucose tolerance,and FBG in the L-Ber group,H-ber group,and Pon group decreased in sequence,the abnormal morphology of the pancreas were reduced,the body weight,HDL-C and the relative expression of p-IRS-1,p-PI3K and GLUT-4 protein increased successively(P<0.05).Conclusion Berberine can improve blood lipid metabolism and IR in DM rats.The mechanism may be related to the regulation of IRS-1/PI3K/GLUT4 pathway.
作者
刘亚慧
郑香梅
李阳
张新丹
侯乾
李红哲
杜杰
王槐向
LIU Yahui;ZHENG Xiangmei;LI Yang;ZHANG Xindan;HOU Qian;LI Hongzhe;DU Jie;WANG Huaixiang(Department of Pharmacy,Xingtai People’s Hospital,Xingtai 054000,China;The Third Department of Gynecology at Xingtai People’s Hospital,Xingtai 054000,China;The Third Department of Neurosurgery at Xingtai People’s Hospital,Xingtai 054000,China;Department of Anesthesiology,Xingtai People’s Hospital,Xingtai 054000,China)
出处
《西北药学杂志》
CAS
2024年第3期65-69,共5页
Northwest Pharmaceutical Journal
基金
2021年度河北省医学科学研究课题计划项目(编号:20210053)
2022年度河北省医学科学研究课题计划项目(编号:20221875)。
关键词
黄连素
糖尿病
血脂代谢
胰岛素抵抗
berberine
diabetes mellitus
lipid metabolism
insulin resistance
