摘要
[目的]探究DKK1与PI3K/AKT信号通路在胃癌细胞的EMT进程中的作用。[方法]免疫组化实验分析胃癌组织以及癌旁正常组织中DKK1的表达水平;将胃癌细胞系MGC-803细胞分为3组:si DKK1组、si NC组和LY294002组。MTT实验分析MGC-803细胞的增殖能力;Transwell实验分析细胞的侵袭能力;细胞划痕实验分析细胞的迁移能力;蛋白免疫印迹实验分析PI3K/AKT信号通路蛋白表达的情况。[结果]与胃癌的癌旁正常组织比较,胃癌组织DKK1的表达水平升高。与si NC组比较,si DKK1和LY294002组的MGC-803细胞生长速度显著下降(2.89±0.02 vs 1.59±0.05 vs 1.37±0.03,P<0.05);si DKK1和LY294002组的MGC-803细胞侵袭数量显著降低(132.23±10.02 vs 71.39±7.05 vs 68.57±8.03,P<0.05);si DKK1和LY294002组的MGC-803细胞迁移比例显著降低(0.83±0.03 vs 0.39±0.01 vs 0.41±0.03,P<0.05);si DKK1和LY294002组的MGC-803细胞PI3K/AKT蛋白磷酸化水平降低(P<0.05)。[结论]DKK1在胃癌组织中高表达,下调DKK1表达后,胃癌MGC-803细胞的增殖、侵袭和迁移能力受到显著抑制,这一过程与下调DKK1抑制PI3K/AKT信号通路激活密切相关。
[Objective]To investigate the role of DKKI and PI3K/AKT signaling pathway in the EMT process of gastric cancer cells.[Method]The expression level of DKKI in gastric cancer tissues and adjacent normal tissues was analyzed by immuno-histochemistry.Gastric cancer cell line MGC-803 cells were divided into three groups:si DKK1 group,si NC group and LY294002 group.The proliferation ability of MGC-803 cells was analyzed by MTT assay.Transwell assay was used to analyze cell invasion ability.The migration ability of cells was analyzed by cell scratch test.The protein expression of PI3K/AKT signa-ling pathway was analyzed by Western Blot.[Result]Compared with the adjacent normal tissues,the expression level of DKKI in gastric cancer tissues was increased.Compared with the si NC group,the growth rate of MGC-803 cells in the si DKK1 and LY294002 groups was significantly decreased(2.89±0.02 us 1.59±0.05 us 1.37±0.03,P<0.05).The number of invasive MCC-803 cells in the si DKK1 and LY294002 groups was significantly decreased(132.23±10.02 us 71.39±7.05 us 68.57±8.03,P<0.05).The migration rate of MCC-803 cells in the si DKK1 and LY294002 groups was significantly decreased(0.83±0.03 us 0.39±0.01 us 0.41±0.03,P<0.05).The phosphorylation level of PI3K/AKT protein in MCC-803 cells was decreased in the si DKKl and LY294002 groups(P<0.05).[Conclusion]DKKI is highly expressed in gastric cancer tissues,and the proliferation,invasion and migration of MGC-803 cells are significantly inhibited by DKKI knockdown,which is closely related to the inhibition of PI3K/AKT signaling pathway activation by DKKI.
作者
宗殿亮
孙清森
赵俊卿
张新如
谷斌
胡广军
ZONG Dianliang;SUN Qingsen;ZHAO Junqing;ZHANG Xinru;GU Bin;HU Guangjun(Department of Gastrointestinal Surgery,Cangzhou People's Hospital,Cangzhou 061000;Department of General Surgery,Cangzhou People's Hospital,Cangzhou 061000;Department of Hospice,Cangzhou People's Hospital,Cangzhou 061000,China)
出处
《生物技术》
CAS
2024年第2期232-236,共5页
Biotechnology
基金
河北省医学科学研究课题计划项目(20220314)。
