摘要
目的：研究循环热灌注化疗对胃癌腹腔积液患者肿瘤标志物含量以及磷脂肌醇3-激酶（PI3K）-蛋白激酶B（Akt）-雷帕霉素靶蛋白（mTOR）（PI3K/Akt/mTOR）通路功能的影响。方法：将2013年5月~2014年8月我院收治的80例胃癌腹腔积液患者纳入研究,随机分为两组,观察组患者接受循环热灌注化疗,对照组接受常规灌注化疗,检测两组患者血液中肿瘤标志物、淋巴细胞活化基因3（LAG-3）和热休克蛋白（HSP）、PI3K-AKT-mTOR信号分子的含量。结果：（1）肿瘤标志物：观察组患者血液中DDK1、EXOSC2含量以及胃蛋白酶原比值（PGR）低于对照组,PGI、PGⅡ高于对照组（P〈0.05）;（2）LAG3和HSP：观察组患者血液中HSP27、HSP90的含量低于对照组,sLAG-3含量高于对照组（P〈0.05）;（3）信号分子：观察组患者血清中PI3K、Akt、mTOR分子的mRNA含量低于对照组（P〈0.05）。结论：循环热灌注化疗有助于杀灭胃癌细胞、降低肿瘤标志物释放入血、调节LAG3和HSP表达、抑制PI3K/Akt/mTOR通路功能,是治疗胃癌腹腔积液的理想方法。
Objective：To study the effects of circulation hyperthermic perfusion chemotherapy on tumor marker content and PI3K/Akt/mTOR pathway function of gastric cancer peritoneal effusion patients.Methods：A total of 80 cases of gastric cancer peritoneal effusion in our hospital from 2013 May to 2014 August were enrolled and randomly divided into two groups.Patients in observation group received circulation hyperthermic perfusion chemotherapy,control group received conventional perfusion chemotherapy.Then blood tumor markers,LAG3 and HSP content,PI3K-AKT-mTOR signal molecules were assayed.Results：DDK1,EXOSC2 contents and PGR ratio of observation group were lower than those of control group;PGI,PGII contents were higher than those of control group.HSP27,HSP90 contents of observation group were lower than those of control group;sLAG-3content was higher than that of control group.mRNA contents of PI3 K,Akt,mTOR molecules of observation group were lower than those of control group.Conclusion：Circulation hyperthermic perfusion chemotherapy is helpful to kill tumor cells,reduce tumor marker releasing into blood,regulate LAG3 and HSP expression,inhibit PI3K/Akt/mTOR pathway function.It＇s an ideal method in treating peritoneal effusion.
出处
《海南医学院学报》
CAS
2015年第9期1268-1270,1274,共4页
Journal of Hainan Medical University
基金
四川省资阳市重点课题(ZNO.WB1032)~~
关键词
胃癌腹腔积液
循环热灌注化疗
肿瘤标志物
热休克蛋白
信号通路
Gastric cancer peritoneal effusion Circulation hyperthermic perfusion chemotherapy Tumor marker Heat shock protein Signal pathway
