摘要
目的建立一种利福喷丁血药浓度检测的二维液相色谱法。方法用新型二维液相色谱仪检测利福喷丁的血药浓度,一维色谱柱:Aston SC2T(3.5 mm×50.0 mm,5μm);二维色谱柱:Aston SCB(4.6 mm×250.0 mm,5μm);柱温:40℃;流速:1.0 mL·min^(-1);检测波长:335 nm;进样体积:300μL。考察该方法的专属性、标准曲线与定量下限、精密度与回收率,及稳定性。用该方法测定结核患者利福喷丁的血药浓度。结果利福喷丁在0.33~18.62μg·mL^(-1)内,线性关系良好,其标准曲线方程为y=2.68×105x-5850.36(r=0.997),回收率为99.81%~105.08%,日内、日间精密度均≤4.84%。结核患者利福喷丁血药浓度测定结果在0.10~54.70μg·mL^(-1),在治疗窗浓度范围(8~30μg·mL^(-1))内的占64.74%。结论该方法操作简便、灵敏度高、检测限低、专属性强,适用于临床血药浓度测定。结核患者服用利福喷丁的个体差异较大,个体化治疗时需要进行血药浓度监测。
Objective To develop a two-dimensional liquid chromatographic method for rifapentine blood concentration determination.Methods The blood concentration of rifapentine was determined by a novel two-dimensional liquid chromatography(2D-LC)with a one-dimensional column:Aston SC2T(3.5 mm×50.0 mm,5μm);a two-dimensional column:Aston SCB(4.6 mm×250.0 mm,5μm);the temperature of the column was 40℃;the flow rate was 1.0 mL·min^(-1);the detection wavelength was 335 nm;the injection volume was 300μL.The specificity,standard curve and lower limit of quantification,precision and recovery,and stability of the method were investigated.The method was used to determine the blood concentration of rifapentine in tuberculosis patients.Results Rifapentine showed good linearity within 0.33-18.62μg·mL^(-1)with the standard curve equation of y=2.68×10~5x-5850.36(r=0.997),the recoveries were 99.81%-105.08%,and the intra-and inter-day precision were≤4.84%.The results of rifapentine blood concentration measurements in tuberculosis patients were in the range of 0.10-54.70μg·mL^(-1),and 64.74%were within the therapeutic window concentration range(8-30μg·mL^(-1)).Conclusion The method is easy to operate,has high sensitivity,low detection limit and high specificity,and is suitable for clinical blood concentration determination.Individual differences in the administration of rifapentine in tuberculosis patients are large,and blood concentration monitoring is required for individualized treatment.
作者
柯涛
何田
张培泽
李巍
周泱
王峰
刘妙娜
KE Tao;HE Tian;ZHANG Pei-ze;LI Wei;ZHOU Yang;WANG Feng;LIU Miao-na(Department of Pharmacy,Shenzhen Third People's Hospital,Shenzhen 518112,Guangdong Province,China;Division Four of Pulmonary Diseases Department,Shenzhen Third People's Hospital,Shenzhen 518112,Guangdong Province,China;Department of Infection and Immunology,Shenzhen Third People's Hospital,Shenzhen 518112,Guangdong Province,China;Department of Pharmacy,The Second Xiangya Hospital of Central South University,Changsha 410012,Hunan Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2024年第11期1638-1641,共4页
The Chinese Journal of Clinical Pharmacology
基金
深圳市科技计划基金资助项目(JCYJ20210324132012035)。
关键词
利福喷丁
肺结核
治疗药物监测
二维液相色谱
个体化用药
rifapentine
phthisis
therapeutic drug monitoring
two-dimensional liquid chromatography
individualized medication
