摘要
目的:通过测量膀胱组织中B细胞淋巴瘤白血病基因(Bcl)-2、Bcl-2-相关X蛋白(Bax)和caspase-3的蛋白和mRNA,以及α-平滑肌肌动蛋白(α-SMA)、转化生长因子(TGF)-β蛋白表达,探讨手捻针针刺对糖尿病神经源性膀胱(DNB)大鼠膀胱细胞凋亡的抑制作用。方法:腹腔注射链脲佐菌素(STZ)建立DNB大鼠模型。大鼠随机分为对照组、模型组和针刺组(MA组),每组10只。针刺组在造模后应用手法针刺。测量大鼠体质量、空腹血糖(FBG)、膀胱湿重,观察膀胱组织形态学;测定膀胱组织中Bcl-2、Bax和caspase-3的蛋白和mRNA,以及α-SMA和TGF-β蛋白的表达水平,并评估膀胱细胞凋亡指数。结果:注射STZ后,与对照组相比,模型组和MA组均从第3周开始显示出较高的FBG,从第9周开始显示出较低的体质量(P<0.05),且膀胱湿重较高(P<0.05)。与模型组比较,MA组显示出较低的膀胱湿重(P<0.05)。组织病理学评估表明,针刺改善了膀胱组织中肌纤维排列紊乱和逼尿肌细胞代偿性肥大。此外,与对照组相比,模型组和MA组凋亡指数均显著升高(P<0.05);模型组和MA组大鼠膀胱组织中Bax和caspase-3蛋白及mRNA、TGF-β蛋白表达量显著升高(P<0.05),大鼠膀胱组织中Bcl-2蛋白及mRNA、α-SMA蛋白表达量显著降低(P<0.05)。与模型组比较,MA组凋亡指数显著降低(P<0.05);大鼠膀胱组织Bax和caspase-3蛋白及mRNA、TGF-β蛋白表达量显著降低(P<0.05);Bcl-2蛋白及mRNA、α-SMA蛋白表达量显著升高(P<0.05)。结论:手捻针针刺可以通过抑制膀胱细胞过度凋亡保护DNB大鼠膀胱,这可能与其下调Bax和caspase-3蛋白和mRNA以及TGF-β蛋白表达,上调Bcl-2蛋白和mRNA以及α-SMA蛋白表达有关。
Objective:To observe the inhibiting effects of manual acupuncture(MA)on bladder cell apoptosis in rats with diabetic neurogenic bladder(DNB)based on the protein and mRNA expression of B-cell lymphoma-leukemia(Bcl)-2,Bcl-2-associated X(Bax)protein,caspase-3,and the protein expression ofα-smooth muscle actin(α-SMA),transforming growth factor(TGF)-βin the bladder tissue.Methods:A DNB rat model was established via intraperitoneal injection of streptozotocin(STZ).The rats were randomly divided into a control group,a model group,and an MA group,with 10 rats in each group.For the MA group,MA was applied after modeling.The body mass,fasting blood glucose(FBG),bladder wet weight,and bladder histomorphology were observed.Protein and mRNA expression levels of Bcl-2,Bax,and caspase-3 and the protein expression ofα-SMA and TGF-βin the bladder tissue were determined.The apoptotic index of bladder cells was also evaluated.Results:After STZ injection,compared with the control group,the model group and the MA group both showed higher FBG from week 3 and lower body mass from week 9(P<0.05),and had a larger bladder wet weight(P<0.05).Compared with the model group,the MA group showed a smaller bladder wet weight(P<0.05).The histopathological evaluation indicated that MA improved muscle fiber alignment and detrusor cell compensatory hypertrophy in the bladder tissue.In addition,compared with the control group,the apoptotic index increased significantly in the model group and the MA group(P<0.05);the protein and mRNA expression levels of Bax and caspase-3 and the protein expression level of TGF-βin the bladder tissue in the model group and the MA group increased significantly(P<0.05),while the protein and mRNA expression levels of Bcl-2 and the protein expression level ofα-SMA in the bladder tissue decreased significantly(P<0.05).Compared with the model group,the apoptotic index of the MA group decreased significantly(P<0.05);the protein and mRNA expression levels of Bax and caspase-3 and the protein expression level of TGF-βi
作者
贺煜竣
苗芙蕊
范郁山
林睿
覃宁婧
张卉
黄敬文
贺彩
HE Yujun;MIAO Furui;FAN Yushan;LIN Rui;QIN Ningjing;ZHANG Hui;HUANG Jingwen;HE Cai(Faculty of Acupuncture,Moxibustion and Tuina,Guangxi University of Chinese Medicine,Nanning 530001,China)
基金
国家自然科学基金项目,No.82260983
广西研究生教育创新计划项目,No.YCBZ2023149,No.YCSW2022351,No.YCSZ2022009
广西壮族自治区中医药管理局中医药人才队伍建设专项范郁山广西名中医传承工作室,No.2023017-05-07.
