摘要
目的:应用改良多平台水环境复合饮食失节法,构建稳定的中枢疲劳肝郁脾虚证病证结合动物模型与评价体系。方法:将30只SPF级雄性Wistar大鼠随机分为空白对照组和模型组。模型组采用水环境小平台站立结合隔日禁食的方法进行造模,空白对照组不作干预措施。对大鼠进行一般情况观察、证候量表评价、行为学评估,对十二指肠、胃、全脑、股四头肌组织进行HE病理染色,检测血清中天冬氨酸转移酶(AST)、丙氨酸转移酶(ALT)、血尿素氮(BUN)、乳酸(LAC)、乳酸脱氢酶(LDH)含量,ELISA法检测血清中醛固酮(ALD)、抗利尿激素(ADH)、血管活性肠肽(VIP)、三磷酸腺苷(ATP)、二磷酸腺苷(ADP)含量以及肝和股四头肌组织中肝糖原及肌糖原含量。结果:与空白对照组比较,模型组出现了生长停滞、精神萎靡、倦怠少动等表现。造模结束后模型组抓握力降低,力竭时间缩短,旷场实验中直立时间、修饰时间、中央区穿格次数百分比、中央区运动时间百分比均降低,高架十字迷宫实验中开放臂进入次数百分比和开放臂停留时间百分比均降低,悬尾实验中挣扎次数减少、不动时间增加,Morris水迷宫实验中逃逸潜伏期增加,穿越平台次数、目标象限游泳时间和目标象限游泳距离百分比均降低,血清中ALT、AST、BUN、LAC、LDH、ALD、ADH、VIP、ADP含量升高,肝糖原、肌糖原和ATP含量降低,粪便含水量增加。模型组大鼠十二指肠、胃、海马CA1区、股四头肌均存在一定病理改变或异常表现。结论:本研究构建的动物模型较为符合中枢疲劳肝郁脾虚证的特点与临床实际,为后续的新药开发及机制研究提供了新的载体。
Objective:Using a modified multi-platform water environment combined with dietary disorder induction method,we established a stable animal model and evaluation system that mimics the syndrome of central fatigue and liver depression combined with spleen deficiency.Methods:Thirty SPF male Wistar rats were randomly allocated into a blank control group and a model group.The model group was induced using a method of standing on a small platform in water combined with fasting every other day,while no intervention measures were applied to the blank control group.General observations,syndrome scale evaluations,behavioral assessments,HE pathological staining of duodenum,stomach,whole brain,and quadriceps muscle tissues,as well as measurements of serum AST,ALT,BUN,LAC,and LDH levels,were carried out.ELISA was used to analyze serum levels of ALD,ADH,VIP,ATP,and ADP,along with hepatic and muscular glycogen content in the liver and muscle tissues.Results:Compared with the blank control group,the model group displayed signs of growth stagnation,mental sluggishness,and decreased activity.After modeling,the model group exhibited reduced grip strength,shortened time to exhaustion,decreased upright time,grooming time,percentage of entries into the central area,percentage of time spent in the central area in the open field test,decreased percentage of entries and time spent in the open arms in the elevated plus maze test,decreased struggling and increased immobility time in the tail suspension test.In the Morris water maze test,the escape latency increased,while the number of platform crossings,time spent in the target quadrant,and distance traveled in the target quadrant all decreased.Serum levels of ALT,AST,BUN,LAC,LDH,ALD,ADH,VIP,and ADP increased,while hepatic glycogen,muscular glycogen,and ATP decreased,and fecal water content increased.Pathological changes or abnormalities were observed in the duodenum,stomach,CA1 region of the hippocampus,and quadriceps muscle of the rats in the model group.Conclusion:The animal model constr
作者
张翼飞
于清茜
张泽涵
兰碧娟
施晴寰
李峰
ZHANG Yifei;YU Qingqian;ZHANG Zehan;LAN Bijuan;SHI Qinghuan;LI Feng(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2024年第5期2174-2180,共7页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金面上项目(No.81874428)
国家自然科学基金青年科学基金项目(No.82204978)。
关键词
中枢疲劳
肝郁脾虚证
动物模型
病证结合
大鼠
Central fatigue
Syndrome of liver depression and spleen deficiency
Animal model
Combination of disease and syndrome
Rat