摘要
采用超声波-盐析辅助水蒸气蒸馏法提取黑胡椒(Piper nigrum L.)精油,利用响应面法确定了最佳的液料比、超声时间、NaCl浓度等提取参数。结果表明,超声波辅助蒸馏法提取黑胡椒精油的最佳条件参数为液料比17∶1、超声时间13 min、NaCl浓度2.4%。在此条件下黑胡椒精油的得率为1.79%。对使用此方法获得的黑胡椒精油进行了抗氧化和抑菌活性检测,结果表明提取物具有一定的抗氧化能力,且对ABTS的清除能力远强于对DPPH的清除能力。同时,提取物对金黄色葡萄球菌和青枯假单胞菌均具有较强的生长抑制功能。
The ultrasonic-assisted salting-out assisted steam distillation method was used to extract essential oil from Piper nigrum L..Response surface methodology was used to determine the optimal extraction parameters such as liquid-solid ratio,ultrasonic time and NaCl concentration.The results showed that the optimal extraction condition of essential oil from Piper nigrum L.by ultrasonic-assist-ed distillation method was the liquid-solid ratio of 17∶1,ultrasonic time of 13 min,and NaCl concentration of 2.4%.Under this condi-tion,the extraction yield of Piper nigrum L.essential oil was 1.79%.The extracts obtained by this method were tested for antioxidant and antibacterial activities.The results showed that Piper nigrum L.essential oil had a certain antioxidant capacity,and its scavenging ability on ABTS was much stronger than that on DPPH.The extract also showed strong growth inhibition against both Staphylococcus aureus and Pseudomonas solanacearum.
作者
谭子祎
曾佳如
李萌
TAN Zi-yi;ZENG Jia-ru;LI Meng(College of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,China)
出处
《湖北农业科学》
2024年第5期162-167,共6页
Hubei Agricultural Sciences
基金
湖南省教育厅重点研究项目(20A521)
湖南省自然科学基金项目(2021JJ31144)
中南林业科技大学校级研究生创新项目(2022CX02068)。
