摘要
通过单因素试验和响应面试验设计,评价浸泡时间、料液比、乙醇浓度、提取温度和提取时间对连翘(Forsythia suspensa)总黄酮提取率的影响,建立数学模型,得出二次回归方程,预测连翘总黄酮提取的最佳工艺条件,并对新工艺提取的连翘总黄酮进行小鼠耳廓急性炎症肿胀抑制效果评价,测试连翘总黄酮提取物的抗炎活性。结果表明,最佳工艺条件为料液比1∶20、浸泡时间30 min、提取温度58℃、乙醇浓度60%、提取时间79 min。经验证,新工艺的平均提取率为14.54%。新工艺提取的连翘总黄酮提取液处理二甲苯急性致炎的小鼠耳廓的肿胀值为(15.61±2.73)mg,低于生理盐水处理后的肿胀值(17.21±2.65)mg,体外抗炎试验表明连翘总黄酮提取物可抑制小白鼠耳廓的炎症肿胀。引入浸泡环节的连翘总黄酮提取工艺具有提取率较高、稳定可靠的特点,新工艺提取的连翘总黄酮具有一定的抗炎效果。
The effects of soaking time,solid-liquid ratio,ethanol concentration,extraction temperature and extraction time on the ex-traction rate of total flavonoids from Forsythia suspensa were evaluated by single factor experiment and response surface experiment de-sign,and a mathematical model was built,which could be used for calculating the quadratic regression equation and predicting the op-timal process of extracting total flavonoids from Forsythia suspensa.The inhibition effect of acute inflammatory swelling of mouse auri-cle with the total flavonoids of Forsythia suspensa extracted by the new process was evaluated,and the anti-inflammatory activity of the total flavonoids of Forsythia suspensa was tested.The results showed that the optimal process condition was solid-liquid ratio of 1∶20,soaking time of 30 min,extraction temperature of 58℃,ethanol concentration of 60%,and extraction time of 79 min.It had been veri-fied that the average extraction rate of the new process was 14.54%.The total flavonoids extract of Forsythia suspensa extracted by the new process had a swelling value of(15.61±2.73)mg of the mouse auricle treated with xylene acute to inflammation,which was lower than the swelling value(17.21±2.65)mg after normal saline treatment.The anti-inflammatory in vitro showed that the extract of total flavonoids from Forsythia suspensa could inhibit inflammatory swelling of the mouse auricle.The extraction process of total flavonoids from Forsythia suspensa with the introduction of soaking process had the characteristics of high extraction rate,stability and reliability,and the Forsythia suspensa total flavonoids extracted by the new process had anti-inflammatory effects.
作者
延永
靳颜颜
张亦琳
程敏
周伟
YAN Yong;JIN Yan-yan;ZHANG Yi-in;CHENG Min;ZHOU Wei(School of Biomedicine and Food Engineering,Shangluo University,Shangluo 726000,Shaanxi,China;Shaanxi Qinling Characteristic Biological Resources Industry Technology Research Institute,Shangluo 726000,Shaanxi,China;Shaanxi Xiangju Pharmaceutical Group Co.,Ltd.,Shangluo 726000,Shaanxi,China)
出处
《湖北农业科学》
2024年第5期156-161,共6页
Hubei Agricultural Sciences
基金
陕西省重点研发项目(2024NC-YBXM-065)
陕西省教育厅2023年度服务地方专项科学研究计划项目(产业化培育项目23JC026)
商洛市科技计划项目(2022-Z0019)
陕西秦岭特色生物资源产业技术研究院2022年度开放基金项目(23HKY033)
陕西省大学生创新创业训练计划项目(S202311396049)。
