摘要
目的探讨lncRNA SLC9A3-AS1在肝细胞癌(HCC)中的表达意义和其参与HCC发生发展的潜在作用机制。方法收集37例HCC组织及其配对癌旁组织,以及HCC细胞RNA,利用qRT-PCR检测lncRNA SLC9A3-AS1在组织和细胞中的表达水平,并分析其与患者临床病理特征的关系。绘制lncRNA SLC9A3-AS1的受试者工作特征(ROC)曲线,分析其对HCC的诊断效能。通过生物信息学方法筛选与lncRNA SLC9A3-AS1结合的RNA结合蛋白,并绘制韦恩图,分析lncRNA SLC9A3-AS1与结合蛋白表达的相关性,以及蛋白表达与HCC病理分期和患者生存预后的关系。结果lncRNA SLC9A3-AS1在HCC组织和细胞中表达下调,其表达水平与患者血清甲胎蛋白(AFP)水平明显相关(P<0.05)。lncRNA SLC9A3-AS1的ROC曲线下面积(AUC)为0.849(95%CI:0.796~0.886)。韦恩图显示5个与lncRNA SLC9A3-AS1潜在结合的蛋白:hnRNPA1、YTHDC1、RBM4、NONO和RBMx,其表达与lncRNA SLC9A3-AS1表达呈明显正相关(P<0.05)。随着HCC分期的进展,不同分期的RNA结合蛋白表达量差异有统计学意义(P<0.05);RBM4、NONO和RBMx低表达患者总体生存期明显长于高表达患者(P<0.05)。结论lncRNA SLC9A3-AS1在HCC中下调,有望作为HCC诊断的生物标志物,并且可能通过与RNA结合蛋白互作调控HCC进展。
Objective To explore the clinical application value of long non-coding RNAs(lncRNA)SLC9A3-AS1 in hepatocellular carcinoma(HCC)and its potential mechanism involved in the occurrence and development of HCC.Methods 37 cases of HCC tissues and their paired paracancerous tissues,as well as HCC cell RNA,were collected.qRT-PCR was used to detect the expression levels of lncRNA SLC9A3-AS1 in tissues and cells,and its relationship with the clinicopathological characteristics of patients was analyzed.A receiver operating characteristic(ROC)curve was generated to assess the diagnostic efficacy of lncRNA SLC9A3-AS1 for HCC.Bioinformatics methods were utilized to identify RNA-binding proteins that interacted with lncRNA SLC9A3-AS1,and Venn diagram was drew to analyze correlation between lncRNA SLC9A3-AS1 and binding protein expression,as well as relationship between protein expression and HCC pathological staging and patient survival prognosis.Results The expression of lncRNA SLC9A3-AS1 was down-regulated in HCC tissues and cells,and its expression level was significantly associated with patients'serum alpha-fetoprotein(AFP)levels(P<0.05).The ROC area under the curve(AUC)of lncRNA SLC9A3-AS1 was 0.849(95%CI:0.796-0.886).The Venn diagram showed 5 proteins potentially binding to lncRNA SLC9A3-AS1:hnRNPA1,YTHDC1,RBM 4,NONO,and RBMx,and their expressions showed a significant positive correlation with the expression of lncRNA SLC9A3-AS1(P<0.05).With the progression of HCC staging,there was a statistically significant difference in RNA binding protein expression levels among different stages(P<0.05).The overall survival time of patients with low expressions of RBM4,NONO,and RBMx was significantly longer than that of patients with high expressions(P<0.05).Conclusion LncRNA SLC9A3-AS1 is down-regulated in HCC and is expected to serve as a biomarker for the diagnosis of HCC,and may regulate HCC progression by interacting with RNA-binding proteins.
作者
蒲俊霞
史俊豪
单杰
邓益斌
PU Junxia;SHI Junhao;SHAN Jie;DENG Yibin(Medical Laboratory Center,the Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Graduate School,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Key Laboratory of Clinical Molecular Diagnosis Research for Diseases with High Incidence of Western Guangxi in Guangxi Higher Education Institutes,Baise 533000,Guangxi,China)
出处
《右江医学》
2024年第5期385-392,共8页
Chinese Youjiang Medical Journal
基金
广西自然科学基金(2018GXNSFAA281187)
广西研究生教育创新项目(YCSW2023495)。
