摘要
目的:探讨芍药苷对多囊卵巢综合征(PCOS)大鼠子宫内膜容受性的改善作用及其可能作用机制。方法:40只雌性大鼠建立PCOS模型,随机分为模型对照组、芍药苷50、100 mg/kg组和阳性对照(达英-350 mg/kg)组,另设正常对照组。各组灌胃相应药物,正常对照组及模型对照组给予等体积生理盐水,连续21 d。ELISA法测定血清中雌二醇(E2)、睾酮(T)、促卵泡生成素(FSH)和促黄体生成素(LH)含量以及子宫内膜组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和IL-6含量;HE染色观察子宫内膜组织病理变化,计算并分析子宫内膜腺体数量、子宫内膜厚度;RT-qPCR法检测子宫内膜组织中Wnt3a和β连环蛋白(Bcatenin)mRNA表达;Western blot法检测子宫内膜组织中Wnt3a、β-catenin、雌激素受体(ER)和孕激素受体(PR)蛋白表达。结果:与正常对照组比较,模型对照组血清E2、FSH、子宫内膜厚度、腺体数量明显降低、子宫内膜组织Wnt3a、Bcatenin mRNA和蛋白表达明显下调,血清T、LH、TNF-α、IL-1β、IL-6含量明显升高、ER和PR蛋白表达明显上调(P<0.05);与模型对照组比较,芍药苷50、100 mg/kg组及达英-350 mg/kg组大鼠血清E2、FSH含量、子宫内膜厚度、腺体数量明显升高,子宫内膜组织Wnt3a、Bcatenin mRNA和蛋白表达明显上调,血清T、LH、TNF-α、IL-1β、IL-6含量明显降低、ER和PR蛋白表达明显下调(P<0.05)。结论:芍药苷可调节PCOS大鼠性激素水平,减轻炎性反应,提高子宫内膜容受性,可能是通过影响Wnt/β-catenin通路发挥作用。
Objective:To investigate the improvement effect and underlying mechanism of paeoniflorin on uterine endometrial receptivity in polycystic ovary syndrome(PCOS)rats.Methods:A PCOS model was induced in 40 female rats and the model rats were randomly divided into a model control group,low-and high-dose paeoniflorin groups(50 and 100 mg/kg),and a positive control group(Diane-35,350 mg/kg),with 10 rats in each group.Another 10 rats were assigned to the normal control group.Each group received the corresponding drugs orally for 21 consecutive days,and the normal control and the model control groups received an equal volume of saline.ELISA was used to measure the serum levels of estradiol(E2),testosterone(T),follicle-stimulating hormone(FSH),and luteinizing hormone(LH),as well as tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and IL-6 in uterine endometrial tissues.Hematoxylin and eosin(HE)staining was performed to observe pathological changes in uterine endometrial tissues and to calculate and analyze the number of uterine endometrial glands and endometrial thickness.RT-qPCR was used to detect the mRNA expression of Wnt3a andβ-catenin in uterine endometrial tissues,and Western blot was employed to determine the protein expression of Wnt3a,β-catenin,estrogen receptor(ER),and progesterone receptor(PR)in uterine endometrial tissues.Results:Compared with the normal control group,the model control group exhibited significantly decreased E2,FSH,uterine endometrial thickness,and the number of uterine endometrial glands,significantly downregulated Wnt3a and β-catenin mRNA and protein expression,significantly elevated T,LH,TNF-α,IL-1β,IL-6 levels,and markedly upregulated ER and PR protein expression(P<0.05).Compared with the model control group,the paeoniflorin groups and the positive control group showed significantly increased E2,FSH,uterine endometrial thickness,and the number of uterine endometrial glands,significantly upregulated Wnt3a andβ-catenin mRNA and protein expression, increased T, LH, TNF-α, IL-1β
作者
李琳
马本玲
贺冰
LI Lin;MA Benling;HE Bing(Department of Gynecology,The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005)
出处
《中药药理与临床》
CAS
CSCD
北大核心
2024年第2期90-95,共6页
Pharmacology and Clinics of Chinese Materia Medica
基金
湖南省自然科学基金项目(编号:2019JJ50459)。
