摘要
选用废弃米糠提取的益生多糖阿拉伯木聚糖(AX)和海藻酸钠(SA)双重微胶囊包埋丁酸梭菌,并在其表面涂层硫酸鱼精蛋白(PRM),提高菌株环境耐受性的同时实现其靶向递送。结果表明:米糠来源的2种AX总糖含量高达(81.86±0.67)%和(76.87±0.12)%,且单糖组成以木糖和阿拉伯糖为主;2种AX能与漆酶作用形成结构密集的网状凝胶;此外,AX还可作为丁酸梭菌利用的益生元,发酵可产生对肠道有益生作用的乳酸、乙酸和丁酸;采用锐孔凝固法结合静电自组装制备得到(SA AX)@PRM复合微胶囊,将其在模拟胃液和高胆盐处理后,存活率相较于游离菌体分别提高了32.4%和16.2%;在模拟消化液释放实验中,(SA AX)@PRM包埋的丁酸梭菌在胃液中保持良好的稳定性,且转移至模拟肠液后可被胰蛋白酶崩解释放,进而实现益生菌在结肠中的靶向释放。
A dual microcapsule system for Clostridium butyricum(C.butyricum)was developed through encapsulation of sodium alginate(SA)with arabinoxylan(AX),which was extracted from discarded rice bran.Such microcapsules could be coated with protamine sulfate(PRM)on their surface to enhance the environmental tolerance for C.butyricum during the targeted delivery.Our research showed the extraction of rice bran afforded two types of AX,which were mainly composed of xylose and arabinose.The total sugar contents turned out to be quite high in both oligosaccharides,reaching(81.86±0.67)%and(76.87±0.12)%,respectively.Further studies revealed that both types of AX could react with laccase to form dense reticular gel.In addition,they could serve as prebiotics for the utilization of C.butyricum,giving fermented products including lactic acid,acetic acid and butyric acid.Based on the above properties,(SA-AX)@PRM dual microcapsules were prepared using piercing-solidifying method by combination with electrostatic self-assembly,then evaluated by treatment with simulated gastric fluid and high bile salt.Compared with free bacterial cells,C.butyricum embedded in(SA-AX)@PRM system displayed higher survival rates under both treatment conditions,providing 32.4%and 16.2%increase,respectively.Finally,a simulated gastrointestinal tract release experiment was conducted to mimic the targeted release of probiotics in the colon.C.butyricum embedded in(SA-AX)@PRM not only demonstrated good stability in the gastric fluid,but also showed the desired release by pancreatic protease upon the transfer to simulated intestinal fluid.
作者
张正怡
王玉先
朱政明
朱丽英
江凌
ZHANG Zhengyi;WANG Yuxian;ZHU Zhengming;ZHU Liying;JIANG Ling(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China;College of Food Science and Light Industry,Nanjing Tech University,Nanjing 211800,China;School of Chemistry and Molecular Engineering,Nanjing Tech University,Nanjing 211800,China;State Key Laboratory of Materials-Oriented Chemical Engineering,Nanjing Tech University,Nanjing 211800,China)
出处
《生物加工过程》
CAS
2024年第3期339-348,共10页
Chinese Journal of Bioprocess Engineering
基金
国家重点研发计划(2021YFC2102700)
国家自然科学基金-山东联合基金重点项目(U2106228)
江苏省先进生物制造创新中心项目(XTC2205)
江苏省高等学校自然科学研究项目资助(23KJB550007)
中国博士后科学基金资助项目(2023M741669)
江苏省“卓博计划”(2023EB025)。
关键词
阿拉伯木聚糖
丁酸梭菌
微胶囊
耐受性
酶响应
arabinoxylan
Clostridium butyricum
microcapsule
environmental tolerance
enzyme-responsive
