摘要
目的探讨妊娠期糖尿病(GDM)糖脂代谢变化与新生儿胎便菌群及免疫功能的关系。方法选取2020年6月至2023年6月于本院产检及分娩的84例GDM患者作为GDM组,另选取同期于本院产检及分娩的84例健康孕妇作为对照组。比较两组的糖脂代谢指标,新生儿胎便菌群的丰富度、均匀度和菌群分布,免疫功能指标水平。采用Pearson相关性分析法分析GDM糖脂代谢指标与新生儿胎便菌群及免疫功能指标的相关性。结果与对照组相比,GDM组空腹血糖(FBG)、餐后2 h血糖(2h PBG)、糖化血红蛋白(HbAlc)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)水平均显著升高(P<0.001),高密度脂蛋白胆固醇(HDL-C)水平显著降低(P<0.001);厚壁菌门相对丰度、Chao1指数、Observed species指数、Shannon指数和Simpson指数均显著降低(P<0.001),变形菌门相对丰度显著升高(P<0.001);GDM组新生儿免疫球蛋白A(IgA)、免疫球蛋白G(IgG)和免疫球蛋白M(IgM)水平,CD3^(+)T淋巴细胞、CD4^(+)T淋巴细胞构成比,CD4^(+)/CD8^(+)比值降低,CD8^(+)T淋巴细胞构成比均升高(P<0.001)。GDM组糖脂代谢指标与新生儿胎便菌群指标、免疫功能指标均具有相关性(P<0.05);新生儿胎便菌群指标与免疫功能指标均具有相关性(P<0.001)。结论GDM患者糖脂代谢紊乱,并影响了新生儿的免疫功能和肠道菌群,且新生儿肠道菌群可影响新生儿的免疫功能。
Objective To explore the relationship of gestational diabetes mellitus (GDM) glycolipid metabolism changes with neonatal meconium microbiota and immune function. Methods Eighty-four GDM patients who underwent prenatal examination and delivery in our hospital from June 2020 to June 2023 were selected as the GDM group, with another 84 healthy pregnant women who underwent prenatal examination and delivery in our hospital during the same period selected as the control group. The glycolipid metabolism indicators, richness, evenness and distribution of neonatal meconium microbiota, and immune function indicators were compared between the two groups. Pearson correlation analysis was used to analyze the correlation between GDM glycolipid metabolism indexes and neonatal meconium microbiota and immune function indicators. Results Compared with the control group, the levels of fasting blood glucose (FBG), 2 h postmeal blood glucose (2 h PBG), glycated hemoglobin (HbAlc), total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) in GDM group significantly increased (P<0.001), while the level of high density lipoprotein cholesterol (HDL-C) significantly decreased (P<0.001);the relative abundance of Firmicutes, Chao1, Observed species, Shannon and Simpson indexes significantly decreased (P<0.001), while the relative abundance of Proteobacteria significantly increased (P<0.001);the levels of immunoglobulin A (IgA), immunoglobulin G (IgG) and immunoglobulin M (IgM), ratio of CD3^(+) T lymphocytes and CD4^(+) T lymphocytes, ratio of CD4^(+)/CD8^(+) decreased, while the ratio of CD8^(+) T lymphocytes increased (P<0.001). In GDM group, the glucose and lipid metabolism indexes were correlated with fetal stool floral indexes and immune function indexes (P<0.05). There was a correlation between fetal stool flora and immune function (P<0.001). Conclusion Glucolipid metabolism disorders in GDM patients may affect the immune function and intestinal flora of newborns, and the intestinal flora of newborns ma
作者
谢建华
XIE Jianhua(Laboratory,the Second People's Hospital of Fuyang District,Hangzhou,Zhejiang 311404,China)
出处
《中国微生态学杂志》
CAS
CSCD
北大核心
2024年第4期467-472,共6页
Chinese Journal of Microecology
关键词
妊娠期糖尿病
糖脂代谢
新生儿
胎便菌群
免疫功能
Gestational diabetes mellitus
Glycolipid metabolism
Neonate
Meconium microbiota
Immune function
