摘要
通过分析不同种植地白木香结香与未结香的根际真菌种群,了解白木香结香前后根际微生物变化规律,为白木香制定合理栽培措施提供参考依据。采用高通量测序技术和生物信息学手段对海南省3个样地(海口文山、临高东光、乐东抱伦)白木香结香前后根际土壤和真菌的多样性及群落结构进行分析,并结合冗余分析阐明白木香结香前后土壤理化性质与真菌群落的相关性。结果表明:3个产地共获得3 697 288条ITS序列,隶属于3个种植区,共获得1界2门12纲25目42科55属62种。样地内结香前后真菌群落和多样性差异性不大,但优势种群丰度存在差异;样地间存在显著差异。白木香结香前后根际土壤真菌丰富度和多样性上从高到低依次呈现为:LDWJX>LDJX>LGJX>LGWJX>WSWJX>WSJX。白木香根际土壤样品真菌物种组成丰富,不同地区白木香根际未结香和结香后土壤真菌群落组成、分类单元的相对丰度及优势分类单元皆存在不同程度差异。在群落结构上,3个产地的优势菌群均存在差异;在门水平上,3个种植区共有的优势菌门为子囊菌门(Ascomycota)、担子菌门(Basidiomycota);在纲水平上,文山优势纲为座囊菌纲(Dothideomycetes),临高优势纲为粪壳菌纲(Sordariomycete),乐东优势纲为子囊菌纲(Ascomycetes);在属水平上,文山优势属为子囊菌属(p__Ascomycota),临高优势属为漆斑菌属(Myrothecium),乐东优势属为座囊菌属(c__Dothideomycetes)。通过对3个种植区白木香根际土壤理化性质与白木香根际真菌的主要菌属进行相关性分析发现,土壤表面pH、有机质、碱解氮、全氮、全磷、有效磷对白木香根际土壤中主要真菌群落有显著影响,表明通过调节土壤养分水平来改变白木香土壤真菌群落组成和遗传多样性。该研究结果对真菌水平上改良土壤和白木香土壤质量评价具有指导意义,也为今后海南省白木香产区的施肥策略提供参�
In this experiment,we analyzed the rhizosphere fungal populations of Aquilaria sinensis(Lour.)Spreng.in dif-ferent planting areas,and understood the change pattern of the rhizosphere microorganisms before and after agarwood in-duction,providing a reference basis for the formulation of reasonable cultivation measures for A.sinensis.High-throughput sequencing and bioinformatics methods were used to analyze the diversity and community structure of the rhizosphere soil and fungi of A.sinensis in three sample sites of Hainan province(Wenshan in Haikou,Dongguang in Lingao,and Baolun in Ledong)before and after agarwood induction,the correlation between soil physicochemical properties and fungal com-munities before and after agarwood induction was also clarified by redundancy analysis.The results indicated that a total of 3697288 ITS sequences belonging to 1 kingdom,2 Phylum,12 classes,9 orders,24 families,55 genera,62 species were obtained from the three planting areas.There was little variability in the fungal communities and diversity before and after agarwood induction in the sample plot,but there was significant differences in the abundance of dominant popula-tion.The Shannon diversity index of the rhizosphere soil fungi of A.sinensis before and after agarwood induction ranged from high to low:LDWJX>LDJX>LGJX>LGWJX>WSWJX>WSJX.The fungal species composition of the rhizosphere soil samples of A.sinensis was rich.There were differences in the soil fungal community composition,the relative abun-dance of taxonomic units and the dominant taxonomic units in the rhizosphere of A.sinensis before and after Agarwood induction in different planting areas.In terms of community structure,there were differences in the dominant populations among the three planting areas;at the Phylum level,the common dominant Phyla in the three cultivation areas were As-comycota and Basidiomycota;at the Class level,the dominant Class in Wenshan was Dothideomycetes,In Lingao the dominant Class was Sordariomycete,In Ledong the dominant Class was Asco
作者
李思
王德立
徐诗涛
张梦真
李慧婷
LI Si;WANG Dei;XU Shitao;ZHANG Mengzhen;LI Huiting(Hainan Branch,Institute of Medicinal Plants,Chinese Academy of Medical Sciences,Haikou,Hainan 570311,China;College of Horticulture,Hainan University,Haikou,Hainan 570228,China;Hainan Lixiangyuan Agroforestry Science and Technology Co.,Ltd.,Ledong,Hainan 572543,China)
出处
《热带作物学报》
CSCD
北大核心
2024年第5期1061-1071,共11页
Chinese Journal of Tropical Crops
基金
海南省自然科学基金高层次人才项目(No.321RC482)
海南省重点研发计划项目(No.ZDYF2022XDNY160)。
关键词
白木香
根际土壤
真菌多样性
理化性质
相关性
Aquilaria sinensis
rhizosphere soil
fungal diversity
physicochemical properties
correlation
