摘要
为寻找结构多样的具有细胞毒活性的真菌代谢物,采用多种色谱分离技术对粉红单端孢霉(Trichothecium roseum)固体发酵代谢产物成分进行分离纯化,得到5个化合物,利用HR-ESI-MS、1H NMR和13 C NMR等谱学技术对其结构进行鉴定,分别确定为melanocane F(1)、rosenonolactone(2)、desoxyrosenonolactone(3)、rosonolactone(4)与2α-hydroxyisocupressic acid(5),均为二萜类化合物,其中化合物1为新化合物。对所有化合物进行乳腺癌MCF-7细胞毒活性研究,结果表明化合物1和5抑制MCF-7生长活性较好。
In order to search for fungal metabolites with various structures and cytotoxic activities,the metabolites of Trichothecium roseum solid fermentation were isolated and purified by various chromatographic techniques.Five diterpenoids were obtained from the fermentation broth of Trichothecium roseum,their structures were identified as one new diterpene compound,melanocane F(1)and four known compounds,rosenonolactone(2),desoxyrosenonolactone(3),rosonolactone(4)and 2α-hydroxyisocupressic acid(5)by the analysis of spectroscopic data including HR-ESI-MS,1H NMR and 13 C NMR.All compounds were evaluated for their cytotoxic activity on MCF-7 cells.As a result,compounds 1 and 5 showed the most significant inhibitory activity on MCF-7 cells.
作者
郝珂冉
黄德森
吴怡婷
邢靓
HAO Ke-ran;HUANG De-sen;WU Yi-ting;XING Liang(Shaanxi Key Laboratory of Natural Products&Chemical Biology,College of Chemistry&Pharmacy,Northwest A&F University,Yangling 712100,China;Guangxi Key Laboratory of Urban Water Environment,College of Chemistry and Environmental Engineering,Baise University,Baise 533000,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2024年第5期793-797,共5页
Natural Product Research and Development
基金
百色市科学研究与技术开发计划(百科20222937)
广西一流学科建设项目(农业资源与环境)。
