摘要
目的通过对金黄色葡萄球菌引起的食物中毒事件进行溯源分析,为流行病学调查和处置提供数据支撑,为临床治疗提供依据。方法2022年9月18日,某区疾控中心接报一行11人在某餐厅就餐后出现呕吐、腹泻、腹痛等症状,疾控中心立即对此次可疑食物中毒事件开展流行病学调查及实验室检测工作,对现场采集的人员、环境标本进行金黄色葡萄球菌分离培养,同时运用MALDI-TOF MS快速鉴定,用PCR法进行肠毒素基因分型;用微量肉汤稀释法开展耐药试验,并通过脉冲场电泳(PFGE)技术溯源分析。结果在此可疑食物中毒事件中采集肛拭子/粪便标本18份、食品4份、环境标本41份,从患者、环境标本分离鉴定到7株金黄色葡萄球菌,除消杀后环境标本未检测到肠毒素,其余患者、环境标本均检测到肠毒素SEA、SEB,分离株对青霉素和达托霉素耐药率高达86%(6/7)、100%(7/7);PFGE结果显示5株病例分离株同源性高达96%。结论此次可疑食物中毒事件由环境污染金黄色葡萄球菌导致,提醒有关部门加强食品风险监管能力,增强公众食品安全意识。
Objective To provide data support for epidemiological investigation and disposal and provide a basis for clinical treatment by tracing the food poisoning caused by Staphylococcus aureus.Methods September 18,2022,a local CDC received a report that 11 people in the same group appeared symptoms such as vomiting,diarrhea and abdominal pain after had dinner at a restaurant.The CDC immediately carried out epidemiological investigation and laboratory testing for this suspected food poisoning incident.Staphylococcus aureus was isolated and cultured from the personnel and environmental samples collected at the scene,MALDI-TOF MS was used for rapid identification,PCR was used for enterotoxin genotyping.Results In this suspected food poisoning incident,a total of 18 anal swab/stool samples,4 food samples and 41 environmental samples were collected,7 strains of Staphylococcus aureus were isolated from patient and environmental samples,and all of them produced SEA and SEB enterotoxins,except for the environmental samples that did not produce enterotoxins after sterilization.The drug resistance rates of isolates to penicillin and daptomycin were 86%(6/7)and 100%(7/7),respectively.PFGE showed that the homology of the 5 isolates was 96%.Conclusion It was showed that this suspicious food poisoning event was caused by environmental contamination of Staphylococcus aureus,which led to the occurrence of food-borne diseases.The relevant departments should strengthen the ability of food risk supervision,and people are called to enhance food safety awareness.
作者
龚玲玉
罗青梅
冯莉
周思雨
殷静
罗益
CONG Lingyu;LUO Qingmei;FENG Li;ZHOU Siyu;YIN Jing;LUO Yi(Microbiology Laboratory,Nan'an District Center for Disease Control and Prevention,Chongqing 401336,China)
出处
《中国国境卫生检疫杂志》
CAS
2024年第2期167-170,共4页
Chinese Journal of Frontier Health and Quarantine
关键词
金黄色葡萄球菌
食源性疾病
脉冲场电泳
耐药试验
Staphylococcus aureus
Food-borne disease
Pulsed field electrophoresis(PFGE)
Drug resistance test
